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RRC ID 88133
著者 Zetka M, Pezeshki A, Dawson R, Huis In 't Veld PJ, Jones SJM, Silva N.
タイトル A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over.
ジャーナル Nat Commun
Abstract The meiotic chromosome axis organizes chromatin and sets the stage for homolog pairing and recombination. Meiotic HORMA domain proteins (mHORMADs) are conserved axis components that conformationally transform during target binding. In C. elegans, four functionally distinct mHORMADs directly interact, but how binding between them is restricted to axis assembly is unknown. Using a mutation in the mHORMADs that delays axis assembly, we isolated a suppressor mutation in a TRiC (Tailless complex peptide 1 Ring Complex) chaperonin subunit that restored mHORMAD localization. CCT-4 associates with meiotic chromatin and forms in vivo complexes with mHORMADs, while germline disruption of TRiC results in axis defects, indicating a nuclear function for TRiC alongside meiotic chromosomes. We propose that chromosome-associated TRiC locally folds mHORMADs into the binding-competent conformation required for axis morphogenesis. More broadly, our results support the model that spatially-restricted folding by TRiC/CCT is a mechanism of controlling the assembly of multimeric complexes that function in tightly co-ordinated events.
巻・号 16(1)
ページ 9411
公開日 2025-10-24
DOI 10.1038/s41467-025-64403-0
PII 10.1038/s41467-025-64403-0
PMID 41136395
PMC PMC12552592
MeSH Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins* / genetics Cell Cycle Proteins* / metabolism Cell Nucleus / metabolism Chaperonin Containing TCP-1* / genetics Chaperonin Containing TCP-1* / metabolism Chromatin / metabolism Chromosomes* / metabolism Crossing Over, Genetic* Meiosis* / genetics Mutation Protein Binding
リソース情報
線虫 tm2543