| RRC ID |
1
|
| 著者 |
Tsuruyama T, Nakamura T, Jin G, Ozeki M, Yamada Y, Hiai H.
|
| タイトル |
Constitutive activation of Stat5a by retrovirus integration in early pre-B lymphomas of SL/Kh strain mice.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
We found that the second intron of Stat5a was one of the common integration sites of the endogenous ecotropic murine leukemia virus, i.e., SL/Kh virus integration-1 (Svi1), in early pre-B lymphomas in SL/Kh mice. The high expression of STAT5A induced by Svi1 integration and activation accelerated the transcription of its target genes such as c-Myc. Transfection of the constitutively active Stat5a mutant cDNA, but not of the wild-type cDNA, to the bone marrow cells induced colony formation of pre-B cells in a methylcellulose medium and escaped from dependence on IL-7. Such growth depended on a genetic factor in the SL/Kh strain. Consitutively high expression of Stat5a either by retrovirus integration or transfection of active mutant cDNA can be lymphomagenic to early pre-B cells in collaboration with a certain genetic background factor of mice.
|
| 巻・号 |
99(12)
|
| ページ |
8253-8
|
| 公開日 |
2002-6-11
|
| DOI |
10.1073/pnas.112202899
|
| PII |
112202899
|
| PMID |
12048235
|
| PMC |
PMC123054
|
| MeSH |
3' Untranslated Regions / genetics
Animals
Base Sequence
Bone Marrow Cells / cytology
Bone Marrow Cells / pathology
Cell Division
DNA Primers
DNA-Binding Proteins / genetics*
Exons
Genes, myc
Introns
Leukemia Virus, Murine / genetics*
Lymphoma, B-Cell / genetics*
Lymphoma, B-Cell / virology
Mice
Mice, Inbred Strains
Milk Proteins*
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
STAT5 Transcription Factor
Trans-Activators / genetics*
Transfection
Virus Integration*
|
| IF |
9.412
|
| 引用数 |
32
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00222 |
