Author Tsuruyama T, Nakamura T, Jin G, Ozeki M, Yamada Y, Hiai H.
Title Constitutive activation of Stat5a by retrovirus integration in early pre-B lymphomas of SL/Kh strain mice.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract We found that the second intron of Stat5a was one of the common integration sites of the endogenous ecotropic murine leukemia virus, i.e., SL/Kh virus integration-1 (Svi1), in early pre-B lymphomas in SL/Kh mice. The high expression of STAT5A induced by Svi1 integration and activation accelerated the transcription of its target genes such as c-Myc. Transfection of the constitutively active Stat5a mutant cDNA, but not of the wild-type cDNA, to the bone marrow cells induced colony formation of pre-B cells in a methylcellulose medium and escaped from dependence on IL-7. Such growth depended on a genetic factor in the SL/Kh strain. Consitutively high expression of Stat5a either by retrovirus integration or transfection of active mutant cDNA can be lymphomagenic to early pre-B cells in collaboration with a certain genetic background factor of mice.
Volume 99(12)
Pages 8253-8
Published 2002-6-11
DOI 10.1073/pnas.112202899
PII 112202899
PMID 12048235
PMC PMC123054
MeSH 3' Untranslated Regions / genetics Animals Base Sequence Bone Marrow Cells / cytology Bone Marrow Cells / pathology Cell Division DNA Primers DNA-Binding Proteins / genetics* Exons Genes, myc Introns Leukemia Virus, Murine / genetics* Lymphoma, B-Cell / genetics* Lymphoma, B-Cell / virology Mice Mice, Inbred Strains Milk Proteins* Phenotype Reverse Transcriptase Polymerase Chain Reaction STAT5 Transcription Factor Trans-Activators / genetics* Transfection Virus Integration*
IF 9.504
Times Cited 29