RRC ID |
1041
|
Author |
Miyagi K, Nakano T, Yagi T, Hanafusa M, Imura S, Honda T, Nakano Y, Sano K.
|
Title |
Survey of Vibrio cholerae O1 and its survival over the winter in marine water of Port of Osaka.
|
Journal |
Epidemiol Infect
|
Abstract |
The survey of Vibrio cholerae O1 in marine area was carried out in the Port of Osaka, Japan in 1987-2001, and 51 V. cholerae O1 strains were isolated. All strains were identified to be of El Tor biotype, Ogawa serotype and classic Ubon Kappa-phage type, and were cholera toxin (CT)-negative and CT gene-negative. In order to clarify certain ecological aspects of V. cholerae O1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-field gel electrophoresis (PFGE) with NotI and SfiI restriction enzymes. We found the indistinguishable strains by DNA analysis using PFGE with strains passed for 1 year, and also found the closely related strains with that passed for 3 and 12 years. Those results indicated that V. cholerae O1 can survive over one winter at least, and that it survives in marine water for a long time by undergoing continuous mutation.
|
Volume |
131(1)
|
Pages |
613-9
|
Published |
2003-8-1
|
DOI |
10.1017/s0950268803008756
|
PMID |
12948359
|
PMC |
PMC2870000
|
MeSH |
Adaptation, Physiological
Cholera / epidemiology*
Climate
DNA, Bacterial / analysis*
Electrophoresis, Gel, Pulsed-Field
Japan
Mutation
Seasons
Serotyping
Survival Analysis
Vibrio cholerae O1 / genetics
Vibrio cholerae O1 / pathogenicity*
Water Microbiology
|
IF |
2.152
|
Times Cited |
3
|
WOS Category
|
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
INFECTIOUS DISEASES
|
Resource |
Pathogenic microorganisms |
V. cholerae O1? |