RRC ID 10745
Author Watanabe S, Wakasugi K.
Title Zebrafish neuroglobin is a cell-membrane-penetrating globin.
Journal Biochemistry
Abstract Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit of heterotrimeric G proteins (Galphai) and acts as a guanine nucleotide dissociation inhibitor (GDI) for Galphai. Recently, we used a protein delivery reagent, Chariot, and demonstrated that the GDI activity of human Ngb is tightly correlated with its neuroprotective activity. In the present study, we found that chimeric ZHHH Ngb, in which module M1 of human Ngb is replaced by that of zebrafish Ngb, protects PC12 cells against oxidative stress-induced cell death even in the absence of Chariot. Using fluorescein isothiocyanate (FITC)-labeled Ngb proteins, we demonstrated that both zebrafish and chimeric ZHHH Ngb can penetrate cell membranes in the absence of Chariot, suggesting that module M1 of zebrafish Ngb can translocate into cells. This is the first report of a native cell-membrane-penetrating globin.
Volume 47(19)
Pages 5266-70
Published 2008-5-13
DOI 10.1021/bi800286m
PMID 18416560
MeSH Amino Acid Sequence Animals Apoptosis Cell Hypoxia Cell Line Cell Membrane Permeability* Conserved Sequence Globins / chemistry Globins / genetics Globins / metabolism* Humans Microscopy, Fluorescence Molecular Sequence Data Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neuroglobin Oxidative Stress Oxygen / metabolism Protein Transport Rats Sequence Alignment Zebrafish / metabolism*
IF 2.865
Times Cited 27
Human and Animal Cells PC-12(RCB0009) HeLa(RCB0007)