RRC ID 10758
著者 Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H.
タイトル Differential recognition of heat shock elements by members of the heat shock transcription factor family.
ジャーナル FEBS J
Abstract Heat shock transcription factor (HSF), an evolutionarily conserved stress response regulator, forms trimers and binds to heat shock element (HSE), comprising at least three continuous inverted repeats of the sequence 5'-nGAAn-3'. The single HSF of yeast is also able to bind discontinuously arranged nGAAn units. We investigated interactions between three human HSFs and various HSE types in vitro, in yeast cells, and in HeLa cells. Human HSF1, a stress-activated regulator, preferentially bound to continuous HSEs rather than discontinuous HSEs, and heat shock of HeLa cells caused expression of reporter genes containing continuous HSEs. HSF2, whose function is implicated in neuronal specification and spermatogenesis, exhibited a slightly higher binding affinity to discontinuous HSEs than did HSF1. HSF4, a protein required for ocular lens development, efficiently recognized discontinuous HSEs in a trimerization-dependent manner. Among four human gamma-crystallin genes encoding structural proteins of the lens, heat-induced HSF1 preferred HSEs on the gammaA-crystallin and gammaB-crystallin promoters, whereas HSF4 preferred HSE on the gammaC-crystallin promoter. These results suggest that the HSE architecture is an important determinant of which HSF members regulate genes in diverse cellular processes.
巻・号 276(7)
ページ 1962-74
公開日 2009-4-1
DOI 10.1111/j.1742-4658.2009.06923.x
PII EJB6923
PMID 19250318
MeSH Binding Sites DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* HeLa Cells Heat Shock Transcription Factors Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism Heat-Shock Response / genetics Heat-Shock Response / physiology Humans Phenotype Promoter Regions, Genetic Regulatory Elements, Transcriptional* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Transcription Factors / genetics Transcription Factors / metabolism* gamma-Crystallins / genetics gamma-Crystallins / metabolism
IF 4.392
引用数 40
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HeLa(RCB0007)