| RRC ID |
10760
|
| 著者 |
Ohnuma K, Hayashi Y, Furue M, Kaneko K, Asashima M.
|
| タイトル |
Serum-free culture conditions for serial subculture of undifferentiated PC12 cells.
|
| ジャーナル |
J Neurosci Methods
|
| Abstract |
PC12 cells, a widely used model neuronal cell line, are usually cultured in serum-supplemented medium. This report describes a serum-free medium for the culture of PC12 cells. PC12 cells grown in the two media types had similar growth rates and released dopamine in response to high potassium-induced calcium elevation. However, the levels of dopamine and of dopamine release in cells cultured in the serum-free medium were less than 10% of that in cells cultured in serum-supplemented medium. Dopamine levels recovered within 10 days if cells were returned to serum-supplemented medium, but dopamine release could not be recovered. Nerve growth factor (NGF) induced similar responses in PC12 cells cultured in both media, including phosphorylation of extracellular signal-regulated protein kinases and neurite extension. Transferrin was necessary for survival of neurite-bearing PC12 cells subcultured in serum-free medium and insulin promoted the cells proliferation. Ten days culture with NGF produced a similar increase in neurofilament expression and acetylcholinesterase activity in both media. These results suggest that PC12 in the hormonally defined serum-free media are qualitatively the same as those cultured in serum-supplemented media, and therefore this new culture protocol should enable more precise studies of PC12 cells culture in the absence of confounding unknown factors.
|
| 巻・号 |
151(2)
|
| ページ |
250-61
|
| 公開日 |
2006-3-15
|
| DOI |
10.1016/j.jneumeth.2005.08.004
|
| PII |
S0165-0270(05)00275-X
|
| PMID |
16169086
|
| MeSH |
Acetylcholinesterase / metabolism*
Animals
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Cell Proliferation
Cell Survival
Culture Media, Serum-Free / metabolism
Dopamine / metabolism*
Nerve Growth Factor / metabolism*
Neurons / cytology*
Neurons / physiology*
PC12 Cells
Rats
|
| IF |
2.214
|
| 引用数 |
14
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
NEUROSCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
