論文 - 詳細
| RRC ID | 10760 |
|---|---|
| 著者 | Ohnuma K, Hayashi Y, Furue M, Kaneko K, Asashima M. |
| タイトル | Serum-free culture conditions for serial subculture of undifferentiated PC12 cells. |
| ジャーナル | J Neurosci Methods |
| Abstract |
PC12 cells, a widely used model neuronal cell line, are usually cultured in serum-supplemented medium. This report describes a serum-free medium for the culture of PC12 cells. PC12 cells grown in the two media types had similar growth rates and released dopamine in response to high potassium-induced calcium elevation. However, the levels of dopamine and of dopamine release in cells cultured in the serum-free medium were less than 10% of that in cells cultured in serum-supplemented medium. Dopamine levels recovered within 10 days if cells were returned to serum-supplemented medium, but dopamine release could not be recovered. Nerve growth factor (NGF) induced similar responses in PC12 cells cultured in both media, including phosphorylation of extracellular signal-regulated protein kinases and neurite extension. Transferrin was necessary for survival of neurite-bearing PC12 cells subcultured in serum-free medium and insulin promoted the cells proliferation. Ten days culture with NGF produced a similar increase in neurofilament expression and acetylcholinesterase activity in both media. These results suggest that PC12 in the hormonally defined serum-free media are qualitatively the same as those cultured in serum-supplemented media, and therefore this new culture protocol should enable more precise studies of PC12 cells culture in the absence of confounding unknown factors. |
| 巻・号 | 151(2) |
| ページ | 250-61 |
| 公開日 | 2006-3-15 |
| DOI | 10.1016/j.jneumeth.2005.08.004 |
| PII | S0165-0270(05)00275-X |
| PMID | 16169086 |
| MeSH | Acetylcholinesterase / metabolism* Animals Cell Culture Techniques / methods* Cell Differentiation Cell Line Cell Proliferation Cell Survival Culture Media, Serum-Free / metabolism Dopamine / metabolism* Nerve Growth Factor / metabolism* Neurons / cytology* Neurons / physiology* PC12 Cells Rats |
| IF | 2.214 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |