論文 - 詳細
| RRC ID | 10774 |
|---|---|
| 著者 | Matsuda H, Akaki J, Nakamura S, Okazaki Y, Kojima H, Tamesada M, Yoshikawa M. |
| タイトル | Apoptosis-inducing effects of sterols from the dried powder of cultured mycelium of Cordyceps sinensis. |
| ジャーナル | Chem Pharm Bull (Tokyo) |
| Abstract |
The methanolic extract and its ethyl acetate-soluble fraction from the dried powder of cultured Cordyceps sinensis mycelium showed cytotoxic effects on promyelocytic leukemia HL-60 cells. Sitosterol (1), 5 alpha,8 alpha-epidioxy-22E-ergosta-6,22-dien-3beta-ol (2), 5 alpha,8 alpha-epidioxy-22E-ergosta-6,9(11),22-trien-3beta-ol (3), 5 alpha,6 alpha-epoxy-5 alpha-ergosta-7,22-dien-3beta-ol (4), and ergosterol (5) were isolated from the ethyl acetate fraction. Among the isolated compounds, 2-4 with a peroxide ring or an epoxide ring showed substantial cytotoxic activity with IC(50) values of 7.3-7.8 microg/ml, but 1 and 5 showed only moderate activity. Furthermore, apoptosis of HL-60 cells was observed 24 h after treatment with 2-4 (10, 20 microg/ml) using the TdT-mediated dUTP nick-end labeling method, and their apoptosis-inducing activities are suggested to be dependent on the activation of caspases-3/7. To the best of our knowledge, this is the first report of the isolation of sterol constituents (1-5) from cultured C. sinensis mycelium. |
| 巻・号 | 57(4) |
| ページ | 411-4 |
| 公開日 | 2009-4-1 |
| DOI | 10.1248/cpb.57.411 |
| PII | JST.JSTAGE/cpb/57.411 |
| PMID | 19336939 |
| MeSH | Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Cordyceps / chemistry* HL-60 Cells Humans Molecular Structure Mycelium / chemistry Powders Sterols / isolation & purification Sterols / pharmacology* |
| IF | 1.416 |
| 引用数 | 22 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |