論文 - 詳細
| RRC ID | 10797 |
|---|---|
| 著者 | Shirogane Y, Takeda M, Tahara M, Ikegame S, Nakamura T, Yanagi Y. |
| タイトル | Epithelial-mesenchymal transition abolishes the susceptibility of polarized epithelial cell lines to measles virus. |
| ジャーナル | J Biol Chem |
| Abstract |
Measles virus (MV), an enveloped negative-strand RNA virus, remains a major cause of morbidity and mortality in developing countries. MV predominantly infects immune cells by using signaling lymphocyte activation molecule (SLAM; also called CD150) as a receptor, but it also infects polarized epithelial cells, forming tight junctions in a SLAM-independent manner. Although the ability of MV to infect polarized epithelial cells is thought to be important for its transmission, the epithelial cell receptor for MV has not been identified. A transcriptional repressor, Snail, induces epithelial-mesenchymal transition (EMT), in which epithelial cells lose epithelial cell phenotypes, such as adherens and tight junctions. In this study, EMT was induced by expressing Snail in a lung adenocarcinoma cell line, II-18, which is highly susceptible to wild-type MV. Snail-expressing II-18 cells lost adherens and tight junctions. Microarray analysis confirmed the induction of EMT in II-18 cells and suggested a novel function of Snail in protein degradation and distribution. Importantly, wild-type MV no longer entered EMT-induced II-18 cells, suggesting that the epithelial cell receptor is down-regulated by the induction of EMT. Other polarized cell lines, NCI-H358 and HT-29, also lost susceptibility to wild-type MV when EMT was induced. However, the complete formation of tight junctions rather reduced MV entry into HT-29 cells. Taken together, these data suggest that the unidentified epithelial cell receptor for MV is involved in the formation of epithelial intercellular junctions. |
| 巻・号 | 285(27) |
| ページ | 20882-90 |
| 公開日 | 2010-7-2 |
| DOI | 10.1074/jbc.M110.102590 |
| PII | S0021-9258(20)57375-4 |
| PMID | 20435897 |
| PMC | PMC2898294 |
| MeSH | Animals Cell Line Disease Susceptibility Epithelial Cells / cytology* Epithelial Cells / physiology Epithelial Cells / virology Flow Cytometry Genetic Predisposition to Disease Genetic Vectors Genome, Viral Humans Lymphocyte Activation Macaca mulatta / virology Measles / immunology Measles / prevention & control* Measles / transmission Measles / veterinary Measles virus / genetics Measles virus / pathogenicity* Membrane Cofactor Protein / physiology Mesoderm / cytology* Mesoderm / physiology Mesoderm / virology Oligonucleotide Array Sequence Analysis Plasmids Receptors, Virus / physiology Tight Junctions / physiology Virus Shedding |
| IF | 4.238 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | II-18(RCB2093) |