| RRC ID |
10801
|
| 著者 |
Higashi T, Miyakawa S, Uchiyama S, Matsunaga S, Takata H, Fujimoto S, Noda M, Terauchi A, Shimizu T, Oda M, Azuma T, Fukui K.
|
| タイトル |
Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse.
|
| ジャーナル |
J Biotechnol
|
| Abstract |
We raised monoclonal antibodies by immunizing mice with total chromosome proteins extracted from isolated human metaphase chromosomes. The indirect immunofluorescence screening of hybridoma cell lines provided 15 monoclonal antibodies against the chromosomal antigens. The antigen proteins of the mAbs were identified by immunoblotting as core histones or by immunoprecipitation followed by a peptide mass fingerprinting method as nuclear mitotic apparatus protein, heterogeneous nuclear ribonucleoprotein A2/B1, ribosomal protein S4, linker histone and beta-actin. During mitosis, localizations of these proteins on chromosomes were clearly observed using the obtained antibodies. These results indicate that the current strategy is effective for obtaining monoclonal antibodies useful for immunoblotting and/or immunofluorescent staining of human proteins, using the antigens with high homology to mouse proteins.
|
| 巻・号 |
120(3)
|
| ページ |
262-72
|
| 公開日 |
2005-11-21
|
| DOI |
10.1016/j.jbiotec.2005.06.036
|
| PII |
S0168-1656(05)00493-1
|
| PMID |
16171883
|
| MeSH |
Animals
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology*
Antibody Specificity*
Antigens / immunology*
Cell Culture Techniques
Cell Line, Tumor
Chromosomal Proteins, Non-Histone / analysis*
Chromosomal Proteins, Non-Histone / immunology
Chromosomes, Human / immunology*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
HeLa Cells
Humans
Hybridomas / immunology
Immunization, Passive
Immunoblotting
Lymphocytes / cytology
Lymphocytes / immunology
Mass Spectrometry
Metaphase
Mice
Multiple Myeloma / immunology
Multiple Myeloma / pathology
Peptide Mapping
Precipitin Tests
|
| IF |
3.503
|
| 引用数 |
2
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
BALL-1(RCB0256) |
