RRC ID 10813
著者 Yamaya M, Nishimura H, Hatachi Y, Yoshida M, Fujiwara H, Asada M, Nakayama K, Yasuda H, Deng X, Sasaki T, Kubo H, Nagatomi R.
タイトル Procaterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells.
ジャーナル Eur J Pharmacol
Abstract β(2) agonists reduce the frequency of exacerbations in patients with bronchial asthma and chronic obstructive pulmonary disease caused by respiratory virus infection. β(2) agonists reduce the production of pro-inflammatory cytokines. However, the inhibitory effects of β(2) agonists on the infection of rhinovirus, the major cause of exacerbations, have not been well studied. To examine the effects of a β(2) agonist, procaterol, on rhinovirus infection and rhinovirus infection-induced airway inflammation, human tracheal epithelial cells were infected with a major group rhinovirus, type 14 rhinovirus. Rhinovirus infection increased viral titers and the content of pro-inflammatory cytokines, including interleukin-1β and interlukin-6, in supernatant fluids and rhinovirus RNA in the cells. Procaterol reduced rhinovirus titers and RNA, cytokine concentrations, and susceptibility to rhinovirus infection. Procaterol reduced the expression of intercellular adhesion molecule-1 (ICAM-1), the receptor for type 14 rhinovirus, and the number of acidic endosomes in the cells from which rhinovirus RNA enters into the cytoplasm. Procaterol inhibited the activation of nuclear factor kappa-B (NF-κB) proteins including p50 and p65 in the nuclear extracts, while it increased the cytosolic amount of the inhibitory kappa B-α and intracellular cyclic AMP (cAMP) levels. A selective β(2)-adrenergic receptor antagonist ICI 118551 [erythro-dl-1-(7-methylindan-4-yloxy)-3-isopropylaminobutan-2-ol] reversed the inhibitory effects of procaterol on rhinovirus titers and RNA, susceptibility to rhinovirus infection, pro-inflammatory cytokines production, ICAM-1 expression, acidic endosomes, and NF-κB. ICI 118551 also reversed the effects of procaterol on cAMP levels. Procaterol may inhibit rhinovirus infection by reducing ICAM-1 and acidic endosomes as well as modulate airway inflammation in rhinovirus infection.
巻・号 650(1)
ページ 431-44
公開日 2011-1-10
DOI 10.1016/j.ejphar.2010.09.056
PII S0014-2999(10)00977-5
PMID 20940011
MeSH Adrenergic beta-2 Receptor Agonists / pharmacology* Cells, Cultured Cyclic AMP / metabolism Cytokines / biosynthesis DNA Replication / drug effects Endosomes / chemistry Endosomes / drug effects Endosomes / virology Epithelial Cells / cytology Epithelial Cells / drug effects* Epithelial Cells / metabolism Epithelial Cells / virology* Gene Expression Regulation / drug effects Humans Hydrogen-Ion Concentration Intercellular Adhesion Molecule-1 / biosynthesis Intercellular Adhesion Molecule-1 / genetics Intracellular Space / drug effects Intracellular Space / metabolism NF-kappa B / metabolism Procaterol / pharmacology* RNA, Viral / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Rhinovirus / drug effects* Rhinovirus / genetics Rhinovirus / metabolism Rhinovirus / physiology* Trachea / cytology*
IF 3.263
引用数 20
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 HFL-III(RCB0523)