RRC ID 1082
Author Abliz P, Fukushima K, Takizawa K, Nishimura K.
Title Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis.
Journal FEMS Immunol. Med. Microbiol.
Abstract The nucleotide sequences of the D1/D2 domains of large subunit (26S) ribosomal DNA for 76 strains of 46 species of pathogenic dematiaceous fungi and related taxa were determined. Intra-species sequence diversity of medically important dematiaceous fungi including Phialophora verrucosa, Fonsecaea pedrosoi, Fonsecaea compacta, Cladophialophora carrionii, Cladophialophora bantiana, Exophiala dermatitidis, Exophiala jeanselmei, Exophiala spinifera, Exophiala moniliae, and Hortaea werneckii were extremely small; as few as 0 changes were detected in C. bantiana, Fonsecaea and Exophiala species, 1 bp in C. carrionii and H. werneckii, and 2 bp in P. verrucosa. Inter-species nucleotide diversity between most species was higher. These data suggested that the D1/D2 domain is sufficiently variable for identification of pathogenic dematiaceous fungi and relevant species. The phylogenetic trees constructed from the sequence data revealed that most human pathogenic species formed a single cluster and that Cladosporium and Phialophora species were distributed polyphyletically into several clusters.
Volume 40(1)
Pages 41-9
Published 2004-1-15
DOI 10.1016/S0928-8244(03)00275-X
PII S092882440300275X
PMID 14734185
MeSH Ascomycota / classification Ascomycota / isolation & purification Cladosporium / classification Cladosporium / isolation & purification DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics* DNA, Ribosomal / isolation & purification Dermatomycoses / microbiology Exophiala / classification Exophiala / isolation & purification Fungi / classification Fungi / isolation & purification* Humans In Vitro Techniques Indicators and Reagents Mitosporic Fungi / classification* Mitosporic Fungi / genetics Mitosporic Fungi / pathogenicity Molecular Sequence Data Mycological Typing Techniques Mycology / methods* Phialophora / classification Phialophora / isolation & purification Phylogeny* Polymorphism, Restriction Fragment Length Sequence Analysis, DNA
Times Cited 56
WOS Category INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY
Resource
Pathogenic microorganisms IFM 4802 IFM 4805 IFM 4808 IFM 4810 IFM 4812 IFM 4818 IFM 4823 IFM 4852 IFM 4856 IFM 4883 IFM 4885 IFM 4886 IFM 4887 IFM 4889 IFM 4914 IFM 4922 IFM 4923 IFM 4924 IFM 4925 IFM 4926 IFM 4928 IFM 4930 IFM 4931 IFM 4974 IFM 40703 IFM 41348 IFM 41433 IFM 41446 IFM 41447 IFM 41451 IFM 41452 IFM 41453 IFM 41454 IFM 41458 IFM 41479 IFM 41500 IFM 41504 IFM 41505 IFM 41538 IFM 41541 IFM 41577 IFM 41578 IFM 41641 IFM 41691 IFM 41704 IFM 41705 IFM 41710 IFM 41818 IFM 41828 IFM 41832 IFM 41871 IFM 41873 IFM 41879 IFM 41898 IFM 41931 IFM 45986 IFM 45989 IFM 46164 IFM 46410 IFM 51361 IFM 51362 IFM 51363 IFM 51364 IFM 51365 IFM 51366 IFM 51367 IFM 51368 IFM 51369 IFM 51370 IFM 51371 IFM 51372 IFM 51373 FM 52022 IFM 52023 IFM 52024 IFM 52025