Reference - Detail
| RRC ID | 10824 |
|---|---|
| Author | Yamaguchi N, Kukita T, Li YJ, Martinez Argueta JG, Saito T, Hanazawa S, Yamashita Y. |
| Title | Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans. |
| Journal | FEMS Immunol Med Microbiol |
| Abstract |
Previous epidemiologic studies have suggested that periodontal disease is closely related to obesity and glucose tolerance. As the level of adiponectin, an adipocyte-derived cytokine, in plasma had been reported to decrease in obese and type 2 diabetes patients, we explored the role of adiponectin in the etiology of periodontitis using the D clone of RAW264, a clone that exhibits highly efficient osteoclast formation, to determine whether adiponectin acts as a regulatory molecule in osteoclast formation stimulated by lipopolysaccharide of periodontopathic bacteria. We observed that adiponectin acted as a potent inhibitor of osteoclast formation stimulated by Toll-like receptor 4 (TLR4) ligand and receptor activator of NF-kappaB ligand (RANKL). Because NF-kappaB is an important transcription factor in osteoclast formation, we examined the effect of adiponectin on its transcriptional activity. A luciferase assay showed that adiponectin was able to inhibit the TLR4-mediated NF-kappaB activity in RAW264 cells. In addition, we observed that the cytokine was actually able to inhibit TLR4-mediated expression of the gene for inducible nitric oxide synthase and production of nitric oxide in the cells. These observations strongly suggest that adiponectin may function as a negative regulator of lipopolysaccharide/RANKL-mediated osteoclast formation in periodontal disease. |
| Volume | 49(1) |
| Pages | 28-34 |
| Published | 2007-2-1 |
| DOI | 10.1111/j.1574-695X.2006.00164.x |
| PII | FIM164 |
| PMID | 17094790 |
| MeSH | Actinobacillus Infections Adiponectin / pharmacology* Aggregatibacter actinomycetemcomitans / chemistry* Animals Cell Line Gene Expression Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology* Macrophages / drug effects Macrophages / metabolism Mice NF-kappa B / metabolism Nitric Oxide / biosynthesis Nitric Oxide Synthase Type II / biosynthesis Nitric Oxide Synthase Type II / genetics Osteoclasts / cytology Osteoclasts / drug effects* Osteoclasts / metabolism Periodontitis / etiology* Periodontitis / microbiology RANK Ligand / pharmacology RNA, Messenger / biosynthesis RNA, Messenger / genetics Signal Transduction / drug effects Toll-Like Receptor 4 / metabolism |
| Times Cited | 77 |
| WOS Category | INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |