論文 - 詳細
| RRC ID | 10825 |
|---|---|
| 著者 | Ishii S, Katsumura T, Shiozuka C, Ooyauchi K, Kawasaki K, Takigawa S, Fukushima T, Tokuji Y, Kinoshita M, Ohnishi M, Kawahara M, Ohba K. |
| タイトル | Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatry effect in mice was observed when ExtBS was treated intraperitoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds. |
| 巻・号 | 72(12) |
| ページ | 3148-57 |
| 公開日 | 2008-12-1 |
| DOI | 10.1271/bbb.80324 |
| PII | JST.JSTAGE/bbb/80324 |
| PMID | 19060399 |
| MeSH | Animals Anti-Inflammatory Agents / administration & dosage Anti-Inflammatory Agents / pharmacology* Cell Line, Tumor Cell Survival / drug effects Colonic Neoplasms / pathology* Fagopyrum / chemistry* Flavonoids / pharmacology Galactosamine / toxicity Gene Expression Regulation, Neoplastic / drug effects Humans Inflammation / chemically induced Inflammation / metabolism Interleukin-8 / genetics Lipopolysaccharides / administration & dosage Lipopolysaccharides / toxicity* Liver / drug effects Liver / injuries Mice Oligonucleotide Array Sequence Analysis Plant Extracts / administration & dosage Plant Extracts / pharmacology* Plant Shoots / chemistry* Reference Standards Up-Regulation / drug effects |
| IF | 1.516 |
| 引用数 | 25 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CoLoTC(RCB1984) LoVo(RCB1639) CACO-2(RCB0988) COLO-320(RCB1193) THP-1(RCB1189) |