論文 - 詳細
RRC ID | 10829 |
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著者 | Fujiyama K, Misaki R, Katsura A, Tanaka T, Furukawa A, Omasa T, Seki T. |
タイトル | N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells. |
ジャーナル | J Biosci Bioeng |
Abstract |
cDNA encoding H- and L-chains from a mouse monoclonal antibody was introduced into tobacco BY2 cells, and the resulting sugar chain structures of plant-produced antibodies were analyzed by a combination of HPLC, exoglucosidase digestion and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The glycan structures determined were Man(5-6)GlcNAc2 (22.3%), GlcNAcMan5GlcNAc2 (3.1%), GlcNAcMan3FucXylGlcNAc2 (24.4%), GlcNAcMan3XylGlcNAc2 (17.8%), Man3FucXylGlcNAc2 (24.3%), and Man3XylGlcNAc2 (8.1%). The major glycan structures of the antibodies produced by transgenic suspension-cultured cells contain typical plant bisecting beta(1,2)-xylose and alpha(1,3)-fucose residues, suggesting the posttranslational modification of a recombinant antibody in the late Golgi apparatus. |
巻・号 | 101(3) |
ページ | 212-8 |
公開日 | 2006-3-1 |
DOI | 10.1263/jbb.101.212 |
PII | S1389-1723(06)70568-0 |
PMID | 16716920 |
MeSH | Animals Antibodies, Monoclonal / chemistry* Base Sequence Carbohydrate Sequence Cloning, Molecular Genes, Plant Glucosidases / metabolism Golgi Apparatus / metabolism Immunoglobulin G / chemistry Mice Molecular Sequence Data Plants / chemistry Plants / metabolism Polysaccharides / chemistry* Tobacco / metabolism* |
IF | 2.366 |
引用数 | 20 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
リソース情報 | |
ヒト・動物細胞 | 3A21(RCB1285) |