RRC ID 10876
著者 Maccarrone G, Kolb N, Teplytska L, Birg I, Zollinger R, Holsboer F, Turck CW.
タイトル Phosphopeptide enrichment by IEF.
ジャーナル Electrophoresis
Abstract In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract.
巻・号 27(22)
ページ 4585-95
公開日 2006-11-1
DOI 10.1002/elps.200600145
PMID 17066382
MeSH Animals Cell Line, Tumor Humans Isoelectric Focusing / methods* Mice Phosphopeptides / isolation & purification* Phosphorylation Proteins / chemistry* Proteins / metabolism Proton-Motive Force Receptor, Insulin / analysis Recombinant Fusion Proteins / analysis Tandem Mass Spectrometry Trypsin / metabolism
IF 3.081
引用数 20
WOS 分野 CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS
リソース情報
ヒト・動物細胞 OHTA(RCB0545)