論文 - 詳細
RRC ID | 10876 |
---|---|
著者 | Maccarrone G, Kolb N, Teplytska L, Birg I, Zollinger R, Holsboer F, Turck CW. |
タイトル | Phosphopeptide enrichment by IEF. |
ジャーナル | Electrophoresis |
Abstract |
In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract. |
巻・号 | 27(22) |
ページ | 4585-95 |
公開日 | 2006-11-1 |
DOI | 10.1002/elps.200600145 |
PMID | 17066382 |
MeSH | Animals Cell Line, Tumor Humans Isoelectric Focusing / methods* Mice Phosphopeptides / isolation & purification* Phosphorylation Proteins / chemistry* Proteins / metabolism Proton-Motive Force Receptor, Insulin / analysis Recombinant Fusion Proteins / analysis Tandem Mass Spectrometry Trypsin / metabolism |
IF | 3.081 |
引用数 | 20 |
WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS |
リソース情報 | |
ヒト・動物細胞 | OHTA(RCB0545) |