論文 - 詳細
| RRC ID | 10911 |
|---|---|
| 著者 | Okamoto H, Takuwa N, Yatomi Y, Gonda K, Shigematsu H, Takuwa Y. |
| タイトル | EDG3 is a functional receptor specific for sphingosine 1-phosphate and sphingosylphosphorylcholine with signaling characteristics distinct from EDG1 and AGR16. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
AGR16/H218/EDG5 and EDG1 are functional receptors for lysosphingolipids, whereas EDG2 and EGD4 are receptors for lysophosphatidic acid (LPA). The present study demonstrates that EDG3, the yet poorly defined member of the EDG family G protein-coupled receptors, shows identical agonist specificity, but distinct signaling characteristics, compared to AGR16 and EDG1. Overexpression of EDG3 conferred a specific [32P]S1P binding, which was displaced by S1P and sphingosylphosphorylcholine (SPC), but not by LPA or other related lipids. In cells overexpressing EDG3, S1P induced inositol phosphate production and [Ca2+]i increase in a manner only partially sensitive to pertussis toxin (PTX), which was similar to the case of AGR16, but quite different from the case of EDG1, in which the S1P-induced responses were totally abolished by PTX. EDG3 also mediated activation of mitogen-activated protein kinase (MAPK) in PTX-sensitive and Ras-dependent manners, as in the cases of EDG1 and AGR16, although EDG3 and EDG1 were more effectively coupled to activation of MAPK, compared to AGR16. Additionally, EDG3 mediated a decrease in cellular cyclic AMP content, like EDG1, but contrasting with AGR16 which mediated an increase in cyclic AMP. These and previous results establish that EDG1, AGR16 and EDG3 comprise the lysosphingolipid receptor subfamily, each showing distinct signaling characteristics. |
| 巻・号 | 260(1) |
| ページ | 203-8 |
| 公開日 | 1999-6-24 |
| DOI | 10.1006/bbrc.1999.0886 |
| PII | S0006-291X(99)90886-0 |
| PMID | 10381367 |
| MeSH | Animals CHO Cells Calcium / metabolism Calcium-Calmodulin-Dependent Protein Kinases / physiology Cricetinae Cyclic AMP / metabolism DNA-Binding Proteins / physiology* Dose-Response Relationship, Drug Humans I-kappa B Proteins* Immediate-Early Proteins / metabolism* Inositol Phosphates / metabolism Lysophospholipids* NF-KappaB Inhibitor alpha Phosphorylcholine / analogs & derivatives* Phosphorylcholine / metabolism Proprotein Convertases* Protein Kinase C / metabolism Receptors, Cell Surface / metabolism* Receptors, G-Protein-Coupled* Receptors, Lysophospholipid Serine Endopeptidases / metabolism Signal Transduction* Sphingosine / analogs & derivatives* Sphingosine / metabolism ras Proteins / metabolism |
| 引用数 | 10 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |