RRC ID 10938
著者 Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H.
タイトル Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells.
ジャーナル J Electron Microsc (Tokyo)
Abstract Autophagy is one of the major processes involved in the degradation of intracellular materials. Here, we examined the potential impact of heavy ion irradiation on the induction of autophagy in irradiated C2C12 mouse myoblasts and their non-targeted bystander cells. In irradiated cells, ultrastructural analysis revealed the accumulation of autophagic structures at various stages of autophagy (i.e. phagophores, autophagosomes and autolysosomes) within 20 min after irradiation. Multivesicular bodies (MVBs) and autolysosomes containing MVBs (amphisomes) were also observed. Heavy ion irradiation increased the staining of microtubule-associated protein 1 light chain 3 and LysoTracker Red (LTR). Such enhanced staining was suppressed by an autophagy inhibitor 3-methyladenine. In addition to irradiated cells, bystander cells were also positive with LTR staining. Altogether, these results suggest that heavy ion irradiation induces autophagy not only in irradiated myoblasts but also in their bystander cells.
巻・号 59(6)
ページ 495-501
公開日 2010-1-1
DOI 10.1093/jmicro/dfq059
PII dfq059
PMID 20685830
MeSH Adenine / analogs & derivatives Adenine / pharmacology Animals Autophagy / drug effects Autophagy / physiology Autophagy / radiation effects* Bystander Effect / radiation effects* Cell Line Heavy Ions* Lysosomes / metabolism Mice Microscopy, Confocal Microtubule-Associated Proteins / metabolism Multivesicular Bodies Myoblasts / radiation effects* Myoblasts / ultrastructure
引用数 13
WOS 分野 MICROSCOPY
リソース情報
ヒト・動物細胞 C2C12(RCB0987)