| RRC ID |
10970
|
| Author |
Nagao K, Ohta T, Hinohara A, Tahara T, Hagiwara T, Maeda Y, Yoneya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M.
|
| Title |
Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-S3, one of which was hematopoiesis supportive (S3-A9) and the other one of which was non-supportive (S3-A7), and we analyzed their gene expression profiles by gene chip analysis. In the present study, we found that Glypican-1 (GPC1) was highly expressed in the supportive subclone AGM-S3-A9. Over-expression of GPC1 in non-supportive cells led to the proliferation of progenitor cells in human cord blood when cocultured with the transfected-stromal cells. Thus, GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis.
|
| Volume |
377(1)
|
| Pages |
205-9
|
| Published |
2008-12-5
|
| DOI |
10.1016/j.bbrc.2008.09.123
|
| PII |
S0006-291X(08)01879-2
|
| PMID |
18840400
|
| MeSH |
Animals
Aorta / cytology
Aorta / embryology
Aorta / metabolism*
Cell Line
Cell Proliferation
Fetal Blood / cytology
Fetal Blood / metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Glypicans / genetics*
Glypicans / physiology
Gonads / cytology
Gonads / embryology
Gonads / metabolism*
Hematopoiesis / genetics*
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / metabolism
Humans
Mesonephros / cytology
Mesonephros / metabolism*
Mice
Stromal Cells / cytology
Stromal Cells / metabolism
Transfection
|
| IF |
2.985
|
| Times Cited |
3
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
OP9(RCB1124) |
