RRC ID 1098
Author Huaman MC, Roncal N, Nakazawa S, Long TT, Gerena L, Garcia C, Solari L, Magill AJ, Kanbara H.
Title Polymorphism of the Plasmodium falciparum multidrug resistance and chloroquine resistance transporter genes and in vitro susceptibility to aminoquinolines in isolates from the Peruvian Amazon.
Journal Am J Trop Med Hyg
Abstract In vitro drug sensitivity to chloroquine (CQ), mefloquine (MQ) and quinine was investigated in 60 culture-adapted Plasmodium falciparum isolates from malaria patients in Padrecocha, a village in the Amazonian Department of Loreto, Peru. All isolates showed resistance to CQ, decreased susceptibility to quinine, and sensitivity to MQ. These isolates were examined for mutations in the P. falciparum multidrug resistance 1 (pfmdr1) and chloroquine resistance transporter (pfcrt) genes previously linked to CQ resistance. The mutations N86Y and D1246Y, two of the five mutations commonly observed in the pfmdr1 gene of CQ-resistant clones, were not found. The pfcrt mutation K76T, associated with CQ resistance, was identified in all the isolates tested. Sequence analysis of codons 72-76 in the pfcrt gene showed the haplotypes SVMNT and CVMNT.
Volume 70(5)
Pages 461-6
Published 2004-5-1
PII 70/5/461
PMID 15155976
MeSH Animals Antimalarials / pharmacology* Chloroquine / pharmacology* Drug Resistance, Multiple Genes, MDR* Genotype Mefloquine / pharmacology Membrane Proteins* / genetics* Membrane Transport Proteins Mutation Parasitic Sensitivity Tests Plasmodium falciparum / drug effects* Plasmodium falciparum / genetics Polymorphism, Genetic* Protozoan Proteins Quinine / pharmacology
IF 2.126
Times Cited 20
Pathogenic microorganisms Pf004(FCR3) Pf006(K1)