| RRC ID |
1099
|
| 著者 |
Tsuboi T, Kaneko O, Cao YM, Tachibana M, Yoshihiro Y, Nagao T, Kanbara H, Torii M.
|
| タイトル |
A rapid genotyping method for the vivax malaria transmission-blocking vaccine candidates, Pvs25 and Pvs28.
|
| ジャーナル |
Parasitol Int
|
| Abstract |
The antigenic diversity observed in many vaccine candidates is one of the difficulties to design effective malaria vaccine. Since it is prerequisite to survey genetic polymorphism of the vaccine candidate antigens for the vaccine development, it is necessary to establish efficient screening method to detect the genetic polymorphism from a large number of samples. Here, we have established efficient polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) method to detect nucleotide diversity of the malaria transmission-blocking vaccine candidates Pvs25 and Pvs28. We can distinguish all 4 haplotypes of Pvs25 by this method. By introducing BsmI-digestion step for Pvs28, we can distinguish 15/16 haplotypes by single electrophoresis. Since this method requires neither sequencing nor radioisotope labeling, it will be easy to transfer the method into a field based high throughput screening of genetic polymorphism.
|
| 巻・号 |
53(3)
|
| ページ |
211-6
|
| 公開日 |
2004-9-1
|
| DOI |
10.1016/j.parint.2004.01.012
|
| PII |
S1383-5769(04)00014-5
|
| PMID |
15468527
|
| MeSH |
Animals
Antigens, Protozoan / genetics*
Antigens, Surface / genetics*
Genotype
Malaria Vaccines / genetics*
Malaria Vaccines / immunology
Malaria, Vivax / parasitology
Malaria, Vivax / prevention & control*
Malaria, Vivax / transmission*
Molecular Sequence Data
Plasmodium vivax / immunology
Polymerase Chain Reaction*
Polymorphism, Genetic
Polymorphism, Single-Stranded Conformational*
Sequence Analysis, DNA
Time Factors
|
| IF |
1.866
|
| 引用数 |
13
|
|
WOS 分野
|
PARASITOLOGY
|
| リソース情報 |
| 病原微生物 |
|
