| RRC ID |
10990
|
| 著者 |
Yamamichi N, Pugdee K, Chang WJ, Lee SY, Yoshinari M, Hayakawa T, Abiko Y.
|
| タイトル |
Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide.
|
| ジャーナル |
Dent Mater J
|
| Abstract |
Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.
|
| 巻・号 |
27(5)
|
| ページ |
744-50
|
| 公開日 |
2008-9-1
|
| DOI |
10.4012/dmj.27.744
|
| PMID |
18972793
|
| MeSH |
3T3 Cells
Animals
Biocompatible Materials / chemistry*
Cell Adhesion / drug effects
Cell Count
Cell Movement / drug effects
Cells, Cultured
Coated Materials, Biocompatible / chemistry*
Electron Probe Microanalysis
Fibronectins / pharmacology*
Gene Expression Regulation / drug effects*
Integrin-Binding Sialoprotein
Mice
Microscopy, Electron, Scanning
Oligonucleotide Array Sequence Analysis
Oligopeptides / pharmacology
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteocalcin / analysis
Polymerase Chain Reaction
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins / analysis
Surface Properties
Time Factors
Titanium / chemistry*
|
| IF |
1.359
|
| 引用数 |
38
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
