| RRC ID |
10994
|
| 著者 |
Hanagata N, Takemura T, Monkawa A, Ikoma T, Tanaka J.
|
| タイトル |
Pre-adsorbed type-I collagen structure-dependent changes in osteoblastic phenotype.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Type-I collagen is the most abundant extracellular matrix in bones and modulates various functions of osteoblasts. We prepared two different structures of type-I collagen on tissue culture grade polystylene (TCPS) surfaces, one is feltwork structure of filamentous molecules from acid solutions (ACs) and the other is network structure of fibrils from neutral solutions (NCs), to examine effects of the structures on the maturation process of osteoblast-like cells. No significant differences of cell proliferation were observed between TCPS and ACs, but NCs delayed the proliferation. In initial cell attachment, the cells on ACs had tense lamellipodia with sharp tips, while those on NCs had loose lamellipodia. No detectable differences in levels of expressed integrin alpha2- and alpha5-subunits were observed between the structures. Although the matrix mineralization in NCs was also delayed in comparison with TCPS and ACs, fully mineralized levels in NCs were the same as those of TCPS and ACs. In addition, although we examined the effects of densities of pre-adsorbed collagen molecules on osteoblast maturation, the effects were less serious than those of the structures. This study suggests that the structures of collagen affect proliferation and mineralization of osteoblast-like cells.
|
| 巻・号 |
344(4)
|
| ページ |
1234-40
|
| 公開日 |
2006-6-16
|
| DOI |
10.1016/j.bbrc.2006.04.021
|
| PII |
S0006-291X(06)00836-9
|
| PMID |
16647688
|
| MeSH |
Animals
Calcification, Physiologic* / genetics
Cell Adhesion
Cell Line
Cell Proliferation*
Collagen Type I / metabolism
Collagen Type I / ultrastructure*
Gene Expression
Mice
Osteoblasts / metabolism
Osteoblasts / ultrastructure*
Phenotype
|
| IF |
2.985
|
| 引用数 |
23
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
