| RRC ID |
11013
|
| Author |
Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J.
|
| Title |
Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells.
|
| Journal |
J Neurosci Res
|
| Abstract |
Recent studies have indicated that embryonic stem cells (ESCs) can be a source for the replacement of spiral ganglion neurons (SGNs), auditory primary neurons, and neurite projections from ESC-derived neurons to auditory sensory epithelia. However, the potential of ESC-derived neurons for synapse formation with auditory hair cells (HCs) has not been elucidated. The present study therefore aimed to examine the ability of ESC-derived neurons to form synaptic connections with HCs in vitro. Mouse ESC-derived neural progenitors expressing enhanced green fluorescence protein (EGFP) were cocultured with explants of cochlea sensory epithelia obtained from postnatal day 3 mice. After a 7-day culture, neurites of ESC-derived neurons predominantly elongated toward inner hair cells (IHCs), which play a crucial role in sound transmission to SGNs. Immunohistochemical analyses revealed the expression of synapsin 1 and synaptophysin in the nerve endings of ESC-derived neurons adjacent to IHCs, indicating the formation of synaptic connections. Transmission electron microscopy demonstrated synaptic contacts between nerve endings of ESC-derived neurons and IHCs. The present findings show that ESC-derived neurons can make synaptic connections with IHCs.
|
| Volume |
86(14)
|
| Pages |
3075-85
|
| Published |
2008-11-1
|
| DOI |
10.1002/jnr.21754
|
| PMID |
18543340
|
| MeSH |
Animals
Cell Communication / physiology*
Coculture Techniques
Embryonic Stem Cells / metabolism
Embryonic Stem Cells / ultrastructure*
Hair Cells, Auditory / ultrastructure*
Immunohistochemistry
Mice
Microscopy, Electron, Transmission
Neurons / metabolism
Neurons / ultrastructure*
Synapses / ultrastructure*
Synapsins / biosynthesis
Synaptophysin / biosynthesis
|
| IF |
4.699
|
| Times Cited |
31
|
|
WOS Category
|
NEUROSCIENCES
|
| Resource |
| Human and Animal Cells |
MC3T3-G2/PA6(RCB1127) |
