RRC ID 11016
Author Sekiya T, Kojima K, Matsumoto M, Kim TS, Tamura T, Ito J.
Title Cell transplantation to the auditory nerve and cochlear duct.
Journal Exp. Neurol.
Abstract We have developed a technique to deliver cells to the inner ear without injuring the membranes that seal the endolymphatic and perilymphatic chambers. The integrity of these membranes is essential for normal hearing, and the technique should significantly reduce surgical trauma during cell transplantation. Embryonic stem cells transplanted at the internal auditory meatal portion of an atrophic auditory nerve migrated extensively along it. Four-five weeks after transplantation, the cells were found not only throughout the auditory nerve, but also in Rosenthal's canal and the scala media, the most distal portion of the auditory nervous system where the hair cells reside. Migration of the transplanted cells was more extensive following damage to the auditory nerve. In the undamaged nerve, migration was more limited, but the cells showed more signs of neuronal differentiation. This highlights an important balance between tissue damage and the potential for repair.
Volume 198(1)
Pages 12-24
Published 2006-3
DOI 10.1016/j.expneurol.2005.11.006
PII S0014-4886(05)00412-7
PMID 16376874
MeSH Animals Cochlear Duct / physiology* Cochlear Nerve / physiology* Cochlear Nerve / ultrastructure Electric Stimulation / methods Embryo, Mammalian Evoked Potentials, Auditory, Brain Stem / physiology Evoked Potentials, Auditory, Brain Stem / radiation effects Green Fluorescent Proteins / metabolism Immunohistochemistry / methods Indoles Male Mice Microscopy, Electron, Transmission / methods Models, Anatomic Quinolines Radiculopathy / pathology Radiculopathy / physiopathology Radiculopathy / surgery Rats Rats, Sprague-Dawley Stem Cell Transplantation* Stem Cells / physiology Stromal Cells / physiology Thiazoles Time Factors Tubulin / metabolism
IF 4.483
Times Cited 40
WOS Category NEUROSCIENCES
Resource
Human and Animal Cells MC3T3-G2/PA6 (RCB1127)