論文 - 詳細
| RRC ID | 11020 |
|---|---|
| 著者 | Kato A, Endo T, Abiko S, Ariga H, Matsumoto K. |
| タイトル | Induction of truncated form of tenascin-X (XB-S) through dissociation of HDAC1 from SP-1/HDAC1 complex in response to hypoxic conditions. |
| ジャーナル | Exp Cell Res |
| Abstract |
XB-S is an amino-terminal truncated protein of tenascin-X (TNX) in humans. The levels of the XB-S transcript, but not those of TNX transcripts, were increased upon hypoxia. We identified a critical hypoxia-responsive element (HRE) localized to a GT-rich element positioned from -1410 to -1368 in the XB-S promoter. Using an electrophoretic mobility shift assay (EMSA), we found that the HRE forms a DNA-protein complex with Sp1 and that GG positioned in -1379 and -1378 is essential for the binding of the nuclear complex. Transfection experiments in SL2 cells, an Sp1-deficient model system, with an Sp1 expression vector demonstrated that the region from -1380 to -1371, an HRE, is sufficient for efficient activation of the XB-S promoter upon hypoxia. The EMSA and a chromatin immunoprecipitation (ChIP) assay showed that Sp1 together with the transcriptional repressor histone deacetylase 1 (HDAC1) binds to the HRE of the XB-S promoter under normoxia and that hypoxia causes dissociation of HDAC1 from the Sp1/HDAC1 complex. The HRE promoter activity was induced in the presence of a histone deacetylase inhibitor, trichostatin A, even under normoxia. Our results indicate that the hypoxia-induced activation of the XB-S promoter is regulated through dissociation of HDAC1 from an Sp1-binding HRE site. |
| 巻・号 | 314(14) |
| ページ | 2661-73 |
| 公開日 | 2008-8-15 |
| DOI | 10.1016/j.yexcr.2008.05.019 |
| PII | S0014-4827(08)00228-0 |
| PMID | 18588874 |
| MeSH | Animals Base Sequence Binding Sites Breast Neoplasms / enzymology Breast Neoplasms / genetics Cell Hypoxia / drug effects Cell Line, Tumor Drosophila Female Gene Expression Regulation, Neoplastic / drug effects Histone Deacetylase 1 Histone Deacetylases / metabolism* Humans Hydroxamic Acids / pharmacology Hypoxia-Inducible Factor 1, alpha Subunit / metabolism Molecular Sequence Data Protein Binding / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Response Elements / genetics Sp1 Transcription Factor / metabolism* Tenascin / genetics* Transcriptional Activation / drug effects |
| IF | 3.383 |
| 引用数 | 8 |
| WOS 分野 | ONCOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | S2(Drosophila)(RCB1153) MCF7 (RCB1904) |