RRC ID 11049
Author Sakudoh T, Nakashima T, Kuroki Y, Fujiyama A, Kohara Y, Honda N, Fujimoto H, Shimada T, Nakagaki M, Banno Y, Tsuchida K.
Title Diversity in copy number and structure of a silkworm morphogenetic gene as a result of domestication.
Journal Genetics
Abstract The carotenoid-binding protein (CBP) of the domesticated silkworm, Bombyx mori, a major determinant of cocoon color, is likely to have been substantially influenced by domestication of this species. We analyzed the structure of the CBP gene in multiple strains of B. mori, in multiple individuals of the wild silkworm, B. mandarina (the putative wild ancestor of B. mori), and in a number of other lepidopterans. We found the CBP gene copy number in genomic DNA to vary widely among B. mori strains, ranging from 1 to 20. The copies of CBP are of several types, based on the presence of a retrotransposon or partial deletion of the coding sequence. In contrast to B. mori, B. mandarina was found to possess a single copy of CBP without the retrotransposon insertion, regardless of habitat. Several other lepidopterans were found to contain sequences homologous to CBP, revealing that this gene is evolutionarily conserved in the lepidopteran lineage. Thus, domestication can generate significant diversity of gene copy number and structure over a relatively short evolutionary time.
Volume 187(3)
Pages 965-76
Published 2011-3
DOI 10.1534/genetics.110.124982
PII genetics.110.124982
Description Y(黄血)の原因遺伝子はCBPである。CBPはカロテノイド結合タンパク質をコードする。p50系統(+Y/+Y)では、CBPのエクソン2が完全に欠失している。
PMID 21242537
PMC PMC3063685
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Bombyx / growth & development* Carotenoids / metabolism* Carrier Proteins / genetics Evolution, Molecular Gene Dosage* Gene Expression / genetics Genetic Variation* Insect Proteins / classification Insect Proteins / genetics* Insect Proteins / metabolism Molecular Sequence Data Morphogenesis / genetics* Phylogeny Retroelements / genetics Sequence Deletion
IF 4.075
Times Cited 11
WOS Category GENETICS & HEREDITY
Resource
Silkworms Samia cynthia ricini Samia cynthia pryeri Antheraea pernyi Antheraea yamamai b10 c04 c05 c10 c11 c12 c15 c21 c23 c44 e09 n16 p22 p50 w06