RRC ID |
11050
|
著者 |
Tatematsu K, Yamamoto K, Uchino K, Narukawa J, Iizuka T, Banno Y, Katsuma S, Shimada T, Tamura T, Sezutsu H, Daimon T.
|
タイトル |
Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects.
|
ジャーナル |
Genes Cells
|
Abstract |
The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors of ommochromes and pteridines are transported by White/Scarlet and White/Brown heterodimers, respectively. The white egg 2 (w-2) mutant of the silkworm, Bombyx mori, has white eggs and eyes because of lack of ommochrome granules in the serosa and eyes. Here, we report that the silkworm w-2 locus encodes an ortholog of Drosophila scarlet. Our results indicate that Bombyx Scarlet forms a heterodimer with Bombyx White to transport ommochrome precursors, suggesting that formation of a White/Scarlet heterodimer and its involvement in the transport of ommochrome precursors are evolutionarily ancient and widely conserved traits in insects. Contrary to dipteran insects, white and scarlet were juxtaposed in a head-to-tail orientation in the silkworm genome, suggesting that the origin of white and scarlet was a tandem duplication of their ancestral transporter gene. In Bombyx, White is also essential for the transport of uric acid in larval epidermis. However, our results suggest that a Bombyx White/Scarlet heterodimer is not involved in this process. Our results emphasize the functional conservation and diversification of half-type ABC transporter families in insects, which may contribute to their extremely diverse color patterns.
|
巻・号 |
16(4)
|
ページ |
331-42
|
公開日 |
2011-4-1
|
DOI |
10.1111/j.1365-2443.2011.01490.x
|
解説 |
Bm-w-2は、ABCトランスポーターfamilyに属するタンパク質をコードする。w-2を保有するe06系統とe15系統では、Bm-w-2のORF内に7bpの欠失が存在する。w-2rを保有するe16系統では、Bm-w-2のORF内に6bpの欠失が存在する。
|
PMID |
21294818
|
MeSH |
ATP-Binding Cassette Transporters / genetics*
Animals
Bombyx / genetics*
Bombyx / metabolism
Cloning, Molecular
DNA Barcoding, Taxonomic
Drosophila melanogaster / genetics*
Drosophila melanogaster / metabolism
Gene Expression Profiling*
Genetic Association Studies
Insecta / genetics
Molecular Sequence Data
Mutation
Phylogeny
Pigmentation / genetics*
|
IF |
1.655
|
引用数 |
39
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
リソース情報 |
カイコ |
p50
e06
e15
e16
t90 |