Reference - Detail
| RRC ID | 11066 |
|---|---|
| Author | Horn T, Sandmann T, Boutros M. |
| Title | Design and evaluation of genome-wide libraries for RNA interference screens. |
| Journal | Genome Biol |
| Abstract |
RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/. |
| Volume | 11(6) |
| Pages | R61 |
| Published | 2010-6-15 |
| DOI | 10.1186/gb-2010-11-6-r61 |
| PII | gb-2010-11-6-r61 |
| PMID | 20550664 |
| PMC | PMC2911109 |
| MeSH | Animals Anopheles / genetics Computational Biology / methods* Drosophila melanogaster / enzymology Drosophila melanogaster / genetics Gene Knockdown Techniques Genome / genetics* Genome, Human / genetics Genomic Library* Humans Phosphoprotein Phosphatases / metabolism Quality Control RNA Interference* RNA, Small Interfering / metabolism Reproducibility of Results Software* Tribolium / genetics |
| IF | 10.806 |
| Times Cited | 51 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| Resource | |
| Drosophila | |