RRC ID 11069
Author Belacortu Y, Weiss R, Kadener S, Paricio N.
Title Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development.
Journal Gene Expr. Patterns
Abstract cabut (cbt) encodes a transcription factor involved in Drosophila dorsal closure (DC), and it is expressed in embryonic epithelial sheets and yolk cell during this process upon activation of the Jun N-terminal kinase (JNK) signaling pathway. Additional studies suggest that cbt may have a role in multiple developmental processes. To analyze Cbt localization through embryogenesis, we generated a Cbt specific antibody that has allowed detecting new Cbt expression patterns. Immunohistochemical analyses on syncytial embryos and S2 cells reveal that Cbt is localized on the surface of mitotic chromosomes at all mitotic phases. During DC, Cbt is expressed in the yolk cell, in epidermal cells and in the hindgut, but also in amnioserosal cells, which also contribute to the process, albeit cbt transcripts were not detected in that tissue. At later embryonic stages, Cbt is expressed in neurons and glial cells in the central nervous system, and is detected in axons of the central and peripheral nervous systems. Most of these expression patterns are recapitulated by GFP reporter gene constructs driven by different cbt genomic regions. Moreover, they have been further validated by immunostainings of embryos from other Drosophila species, thus suggesting that Cbt function during embryogenesis appears to be conserved in evolution.
Volume 11(3-4)
Pages 190-201
Published 2011-3
DOI 10.1016/j.gep.2010.11.004
PII S1567-133X(10)00119-5
PMID 21109026
MeSH Animals Cell Nucleus / metabolism Central Nervous System / cytology Central Nervous System / embryology Central Nervous System / metabolism Chromatin / metabolism Drosophila / embryology* Drosophila / metabolism Drosophila Proteins / immunology Drosophila Proteins / metabolism* Epidermis / cytology Epidermis / embryology* Epidermis / metabolism Gene Expression Regulation, Developmental Genes, Reporter Laminin / metabolism Mitosis Organ Specificity Peripheral Nervous System / cytology Peripheral Nervous System / embryology Peripheral Nervous System / metabolism Promoter Regions, Genetic Protein Structure, Tertiary Recombinant Fusion Proteins / immunology Transcription Factors / immunology Transcription Factors / metabolism* Tubulin / metabolism
IF 1.515
Times Cited 5
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
Resource
Drosophila 4427R-2