論文 - 詳細
| RRC ID | 11090 |
|---|---|
| 著者 | Karam CS, Kellner WA, Takenaka N, Clemmons AW, Corces VG. |
| タイトル | 14-3-3 mediates histone cross-talk during transcription elongation in Drosophila. |
| ジャーナル | PLoS Genet |
| Abstract |
Post-translational modifications of histone proteins modulate the binding of transcription regulators to chromatin. Studies in Drosophila have shown that the phosphorylation of histone H3 at Ser10 (H3S10ph) by JIL-1 is required specifically during early transcription elongation. 14-3-3 proteins bind H3 only when phosphorylated, providing mechanistic insights into the role of H3S10ph in transcription. Findings presented here show that 14-3-3 functions downstream of H3S10ph during transcription elongation. 14-3-3 proteins localize to active genes in a JIL-1-dependent manner. In the absence of 14-3-3, levels of actively elongating RNA polymerase II are severely diminished. 14-3-3 proteins interact with Elongator protein 3 (Elp3), an acetyltransferase that functions during transcription elongation. JIL-1 and 14-3-3 are required for Elp3 binding to chromatin, and in the absence of either protein, levels of H3K9 acetylation are significantly reduced. These results suggest that 14-3-3 proteins mediate cross-talk between histone phosphorylation and acetylation at a critical step in transcription elongation. |
| 巻・号 | 6(6) |
| ページ | e1000975 |
| 公開日 | 2010-6-3 |
| DOI | 10.1371/journal.pgen.1000975 |
| PMID | 20532201 |
| PMC | PMC2880557 |
| MeSH | 14-3-3 Proteins / metabolism* Acetylation Animals Chromosomes / genetics Chromosomes / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation Histone Acetyltransferases / metabolism Histones / metabolism* Nerve Tissue Proteins / metabolism Phosphorylation Protein Binding Protein Processing, Post-Translational Protein Serine-Threonine Kinases / metabolism Transcription, Genetic* |
| IF | 5.175 |
| 引用数 | 39 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | 31196R-4 |