RRC ID 11098
著者 Berry B, Deddouche S, Kirschner D, Imler JL, Antoniewski C.
タイトル Viral suppressors of RNA silencing hinder exogenous and endogenous small RNA pathways in Drosophila.
ジャーナル PLoS One
Abstract BACKGROUND:In plants and insects, RNA interference (RNAi) is the main responder against viruses and shapes the basis of antiviral immunity. Viruses counter this defense by expressing viral suppressors of RNAi (VSRs). While VSRs in Drosophila melanogaster were shown to inhibit RNAi through different modes of action, whether they act on other silencing pathways remained unexplored.
METHODOLOGY/PRINCIPAL FINDINGS:Here we show that expression of various plant and insect VSRs in transgenic flies does not perturb the Drosophila microRNA (miRNA) pathway; but in contrast, inhibits antiviral RNAi and the RNA silencing response triggered by inverted repeat transcripts, and injection of dsRNA or siRNA. Strikingly, these VSRs also suppressed transposon silencing by endogenous siRNAs (endo-siRNAs).
CONCLUSIONS/SIGNIFICANCE:Our findings identify VSRs as tools to unravel small RNA pathways in insects and suggest a cosuppression of antiviral RNAi and endo-siRNA silencing by viruses during fly infections.
巻・号 4(6)
ページ e5866
公開日 2009-6-10
DOI 10.1371/journal.pone.0005866
PMID 19516905
PMC PMC2689938
MeSH Animals Animals, Genetically Modified Antiviral Agents / pharmacology Crosses, Genetic Drosophila melanogaster / virology* Gene Silencing Heterozygote Photoreceptor Cells, Invertebrate RNA Interference* RNA, Double-Stranded / chemistry Reverse Transcriptase Polymerase Chain Reaction Transgenes
IF 2.74
引用数 51
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ