RRC ID 11118
著者 Kpebe A, Rabinow L.
タイトル Dissection of darkener of apricot kinase isoform functions in Drosophila.
ジャーナル Genetics
Abstract The Darkener of apricot (Doa) locus of Drosophila encodes a LAMMER protein kinase affecting alterative splicing, and hence sex determination, via the phosphorylation of SR and SR-like proteins. Doa encodes 6 different kinases via alternative promoter usage. To provide further insight into the roles of the multiple isoforms, we mapped polymorphisms, deletions, and P-element insertions in the locus, identifying several that are largely, if not completely, isoform specific in their effects. These tests, along with the use of lines permitting overexpression and interfering RNA expression, demonstrate that the major isoforms of 55 and 105 kDa perform separate functions. The 105-kDa and a minor 138-kDa isoform are both vital but do not apparently perform functions essential for sex determination. Curiously, male-specific lethality induced by overexpression of the 55-kDa kinase in the larval fat body is rescued by coexpression of TRA, suggesting a sex-specific physiological role for this isoform. Maternal effects in which the survival of heteroallelic adults depends upon the direction of the cross are consistent with a role for a 105-kDa cytoplasmic kinase in oogenesis or early larval development.
巻・号 179(4)
ページ 1973-87
公開日 2008-8-1
DOI 10.1534/genetics.108.087858
PII genetics.108.087858
PMID 18660538
PMC PMC2516073
MeSH Alleles Animals DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila / embryology Drosophila / enzymology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Embryo, Nonmammalian / metabolism Female Gene Expression Regulation, Developmental Larva / metabolism Male Molecular Sequence Data Oogenesis Phenotype Protein Isoforms / genetics Protein Isoforms / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / physiology* Transcription Factors / genetics Transcription Factors / metabolism
IF 4.015
引用数 11
WOS 分野 GENETICS & HEREDITY
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