RRC ID 11127
著者 Yoshida H, Fuwa TJ, Arima M, Hamamoto H, Sasaki N, Ichimiya T, Osawa K, Ueda R, Nishihara S.
タイトル Identification of the Drosophila core 1 beta1,3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes.
ジャーナル Glycobiology
Abstract T antigen (Galbeta1-3GalNAcalpha1-Ser/Thr), the well-known tumor-associated antigen, is a core 1 mucin-type O-glycan structure that is synthesized by core 1 beta1,3-galactosyltransferase (C1beta3GalT), which transfers Gal from UDP-Gal to Tn antigen (GalNAcalpha1-Ser/Thr). Three putative C1beta3GalTs have been identified in Drosophila. However, although all three are expressed in embryos, their roles during embryogenesis have not yet been clarified. In this study, we used P-element inserted mutants to show that CG9520, one of the three putative C1beta3GalTs, synthesizes T antigen expressed on the central nervous system (CNS) during embryogenesis. We also found that T antigen was expressed on a subset of the embryonic hemocytes. CG9520 mutant embryos showed the loss of T antigens on the CNS and on a subset of hemocytes. Then, the loss of T antigens was rescued by precise excision of the P-element inserted into the CG9520 gene. Our data demonstrate that T antigens expressed on the CNS and on a subset of hemocytes are synthesized by CG9520 in the Drosophila embryo. In addition, we found that the number of circulating hemocytes was reduced in third instar larvae of CG9520 mutant. We, therefore, named the CG9520 gene Drosophila core 1 beta1,3-galactosyltransferase 1 because it is responsible for the synthesis and function of T antigen in vivo.
巻・号 18(12)
ページ 1094-104
公開日 2008-12-1
DOI 10.1093/glycob/cwn094
PII cwn094
PMID 18824562
MeSH Animals Antigens, Tumor-Associated, Carbohydrate / biosynthesis* Antigens, Tumor-Associated, Carbohydrate / immunology Central Nervous System / metabolism Drosophila / embryology* Drosophila / enzymology* Drosophila / genetics Drosophila Proteins / genetics* Embryo, Nonmammalian / enzymology Galactosyltransferases / genetics* Hemocytes / metabolism* Histocytochemistry Mutation Phylogeny
IF 4.06
引用数 20
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ DGRC#108992