論文 - 詳細
| RRC ID | 11151 |
|---|---|
| 著者 | Furuhashi H, Nakajima M, Hirose S. |
| タイトル | DNA supercoiling factor contributes to dosage compensation in Drosophila. |
| ジャーナル | Development |
| Abstract |
DNA supercoiling factor (SCF) is a protein capable of generating negative supercoils in DNA in conjunction with topoisomerase II. To clarify the biological functions of SCF, we introduced a heritable SCF RNAi into Drosophila. Upon knockdown of SCF, we observed male lethality and male-specific reduction in the expression levels of X-linked genes. SCF functionally interacts with components of the MSL complex, which are required for dosage compensation via hypertranscription of the male X chromosome. Moreover, SCF colocalizes with the MSL complex along the male X chromosome. Upon overexpression of SCF, the male X chromosome had a bloated appearance. This phenotype was dependent on the histone acetyltransferase MOF and was suppressed by simultaneous overexpression of ISWI. These findings demonstrate that SCF plays a role in transcriptional activation via alteration of chromatin structure and provide evidence that SCF contributes to dosage compensation. |
| 巻・号 | 133(22) |
| ページ | 4475-83 |
| 公開日 | 2006-11-1 |
| DOI | 10.1242/dev.02620 |
| PII | dev.02620 |
| PMID | 17035293 |
| MeSH | Adenosine Triphosphatases / metabolism Animals Calcium-Binding Proteins / genetics Calcium-Binding Proteins / physiology* Chromatin / genetics Chromatin / metabolism* DNA Primers Dosage Compensation, Genetic / genetics Dosage Compensation, Genetic / physiology* Drosophila / genetics* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Gene Expression Regulation* Genes, X-Linked / genetics* Histone Acetyltransferases / metabolism Immunoblotting Male Nuclear Proteins / genetics Nuclear Proteins / metabolism Nuclear Proteins / physiology* RNA Interference Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / metabolism X Chromosome / genetics X Chromosome / metabolism* |
| IF | 5.611 |
| 引用数 | 32 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | |