RRC ID 11162
Author Ishimoto H, Takahashi K, Ueda R, Tanimura T.
Title G-protein gamma subunit 1 is required for sugar reception in Drosophila.
Journal EMBO J.
Abstract Though G-proteins have been implicated in the primary step of taste signal transduction, no direct demonstration has been done in insects. We show here that a G-protein gamma subunit, Ggamma1, is required for the signal transduction of sugar taste reception in Drosophila. The Ggamma1 gene is expressed mainly in one of the gustatory receptor neurons. Behavioral responses of the flies to sucrose were reduced by the targeted suppression of neural functions of Ggamma1-expressing cells using neural modulator genes such as the modified Shaker K+ channel (EKO), the tetanus toxin light chain or the shibire (shi(ts1)) gene. RNA interference targeting to the Ggamma1 gene reduced the amount of Ggamma1 mRNA and suppressed electrophysiological response of the sugar receptor neuron. We also demonstrated that responses to sugars were lowered in Ggamma1 null mutant, Ggamma1(N159). These results are consistent with the hypothesis that Ggamma1 participates in the signal transduction of sugar taste reception.
Volume 24(18)
Pages 3259-65
Published 2005-9-21
DOI 10.1038/sj.emboj.7600796
PII 7600796
PMID 16121192
PMC PMC1224686
MeSH Animals Behavior, Animal / drug effects Behavior, Animal / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / drug effects* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* GTP-Binding Protein gamma Subunits / genetics GTP-Binding Protein gamma Subunits / metabolism* Gene Expression Homozygote Mutation / genetics Neurons / drug effects Neurons / metabolism Potassium Channels / genetics Potassium Channels / metabolism RNA Interference Shaker Superfamily of Potassium Channels Sucrose / pharmacology* Taste / drug effects Taste / physiology*
IF 10.557
Times Cited 30
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Drosophila 8261R-1 8261R-2