RRC ID 11162
著者 Ishimoto H, Takahashi K, Ueda R, Tanimura T.
タイトル G-protein gamma subunit 1 is required for sugar reception in Drosophila.
ジャーナル EMBO J
Abstract Though G-proteins have been implicated in the primary step of taste signal transduction, no direct demonstration has been done in insects. We show here that a G-protein gamma subunit, Ggamma1, is required for the signal transduction of sugar taste reception in Drosophila. The Ggamma1 gene is expressed mainly in one of the gustatory receptor neurons. Behavioral responses of the flies to sucrose were reduced by the targeted suppression of neural functions of Ggamma1-expressing cells using neural modulator genes such as the modified Shaker K+ channel (EKO), the tetanus toxin light chain or the shibire (shi(ts1)) gene. RNA interference targeting to the Ggamma1 gene reduced the amount of Ggamma1 mRNA and suppressed electrophysiological response of the sugar receptor neuron. We also demonstrated that responses to sugars were lowered in Ggamma1 null mutant, Ggamma1(N159). These results are consistent with the hypothesis that Ggamma1 participates in the signal transduction of sugar taste reception.
巻・号 24(18)
ページ 3259-65
公開日 2005-9-21
DOI 10.1038/sj.emboj.7600796
PII 7600796
PMID 16121192
PMC PMC1224686
MeSH Animals Behavior, Animal / drug effects Behavior, Animal / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / drug effects* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* GTP-Binding Protein gamma Subunits / genetics GTP-Binding Protein gamma Subunits / metabolism* Gene Expression Homozygote Mutation / genetics Neurons / drug effects Neurons / metabolism Potassium Channels / genetics Potassium Channels / metabolism RNA Interference Shaker Superfamily of Potassium Channels Sucrose / pharmacology* Taste / drug effects Taste / physiology*
IF 9.889
引用数 33
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 8261R-1 8261R-2 DGRC#104021