論文 - 詳細
| RRC ID | 11180 |
|---|---|
| 著者 | Lehane DB, McKie N, Russell RG, Henderson IW. |
| タイトル | Cloning of a fragment of the osteonectin gene from goldfish, Carassius auratus: its expression and potential regulation by estrogen. |
| ジャーナル | Gen Comp Endocrinol |
| Abstract |
During reproduction, female teleost fish display increased plasma estrogen and greatly increased total plasma calcium concentrations; the main source of this calcium seems to be the scale. Osteonectin, a collagen-binding glycoprotein, is a major noncollagenous constituent of mammalian bone and is a product mainly of the osteoblasts. RT-PCT has been applied to clone and sequence part of the osteonectin gene from the goldfish, Carassius auratus. The use of a goldfish scale cell line (GFS) and a specific probe to goldfish osteonectin mRNA has allowed the study of the potential effects of estrogen and other calcitropic hormones on the cells derived from the scales. Osteonectin mRNA was detected in teleost bone, scale, and GFS cells by Northern blot analysis, hybridising to a transcript of approximately 1.6 kb. Expression of osteonectin mRNA was markedly down-regulated by 17beta-estradiol (10(-8) to 10(-11) M) in a dose-dependent fashion but was unaffected by calcitriol, TGFbeta, IL-1beta, calcitonin, and PTHrP. Down-regulation of osteonectin by estrogen is further evidence that estrogen participates in calcium homeostasis during vitellogenesis, acting directly on the cells responsible for matrix and mineral fluxes in scales. |
| 巻・号 | 114(1) |
| ページ | 80-7 |
| 公開日 | 1999-4-1 |
| DOI | 10.1006/gcen.1998.7237 |
| PII | S0016-6480(98)97237-9 |
| PMID | 10094861 |
| MeSH | Amino Acid Sequence Animals Blotting, Northern / veterinary Bone and Bones / chemistry Calcitriol / pharmacology Cattle Chickens Cloning, Molecular DNA, Complementary / chemistry Estradiol / pharmacology Estrogens / physiology* Female Gene Expression Regulation Goldfish / genetics* Goldfish / physiology Humans Interleukin-1 / pharmacology Molecular Sequence Data Osteonectin / genetics* Parathyroid Hormone-Related Protein Polymerase Chain Reaction / veterinary Proteins / pharmacology Transforming Growth Factor beta / pharmacology Xenopus laevis |
| 引用数 | 11 |
| WOS 分野 | DERMATOLOGY MYCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | GAKS(RCB0082) |