論文 - 詳細
| RRC ID | 11188 |
|---|---|
| 著者 | Tsujigiwa H, Nagatsuka H, Gunduz M, Rodriguez A, Rivera RS, Legeros RZ, Inoue M, Nagai N. |
| タイトル | Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity. |
| 巻・号 | 75(1) |
| ページ | 210-5 |
| 公開日 | 2005-10-1 |
| DOI | 10.1002/jbm.a.30416 |
| PMID | 16059889 |
| MeSH | Alkaline Phosphatase / chemistry Alkaline Phosphatase / metabolism Animals Biocompatible Materials / chemistry* Blotting, Western Bone Marrow Cells / cytology Bone Marrow Cells / drug effects* Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / chemistry* Bone Morphogenetic Proteins / pharmacology Bone and Bones / metabolism Cell Line Collagen / chemistry* Collagen Type I / chemistry Diffusion Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Humans Mice Recombinant Proteins / chemistry* Recombinant Proteins / pharmacology Succinates / chemistry Time Factors Transforming Growth Factor beta / chemistry* Transforming Growth Factor beta / pharmacology |
| IF | 3.525 |
| 引用数 | 15 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |