論文 - 詳細
| RRC ID | 11199 |
|---|---|
| 著者 | Schreiber O, Petersson J, Phillipson M, Perry M, Roos S, Holm L. |
| タイトル | Lactobacillus reuteri prevents colitis by reducing P-selectin-associated leukocyte- and platelet-endothelial cell interactions. |
| ジャーナル | Am J Physiol Gastrointest Liver Physiol |
| Abstract |
Recent findings indicate that dextran sodium sulfate (DSS)-induced colitis is associated with a prothrombogenic phenotype, with P-selectin playing a major role in platelet recruitment. It has been suggested that probiotics may ameliorate colonic inflammation. We therefore investigated how treatment with Lactobacillus reuteri influenced P-selectin expression, leukocyte and platelet endothelial cell interactions, and colitis severity in DSS-treated rats. Rats were divided into the following four groups: nontreated, DSS treated (5% in drinking water for 9 days), L. reuteri, and L. reuteri and DSS treated. The rats were anesthetized with Inactin (120 mg/kg ip), and the dual radiolabeled monoclonal antibody technique was used to quantify P-selectin expression. Leukocyte-endothelial and platelet-endothelial cell interactions were studied in colonic venules with intravital microscopy. Colitis severity was assessed using a disease activity index. Disease activity index increased, as did the expression of P-selectin in the entire colon after DSS treatment, but both were reduced to control levels with L. reuteri pretreatment. The increased platelet- and leukocyte-endothelial cell interactions after DSS treatment were abolished by pretreatment with L. reuteri. L. reuteri protects against DSS-induced colitis in rats. The protection is associated with reduced P-selectin expression and a decrease in leukocyte- and platelet-endothelial cell interactions. |
| 巻・号 | 296(3) |
| ページ | G534-42 |
| 公開日 | 2009-3-1 |
| DOI | 10.1152/ajpgi.90470.2008 |
| PII | 90470.2008 |
| PMID | 19147805 |
| MeSH | Animals Blood Platelets / cytology Blood Platelets / metabolism Cell Communication / physiology* Colitis / pathology Colitis / prevention & control* Endothelial Cells / cytology Endothelial Cells / metabolism Intestinal Mucosa / metabolism Intestinal Mucosa / pathology Leukocytes / cytology Leukocytes / metabolism Limosilactobacillus reuteri* Male Microscopy, Video P-Selectin / metabolism* Probiotics / pharmacology* Rats Rats, Sprague-Dawley |
| IF | 3.725 |
| 引用数 | 36 |
| WOS 分野 | PHYSIOLOGY GASTROENTEROLOGY & HEPATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 50 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 5869 |