| RRC ID |
11199
|
| Author |
Schreiber O, Petersson J, Phillipson M, Perry M, Roos S, Holm L.
|
| Title |
Lactobacillus reuteri prevents colitis by reducing P-selectin-associated leukocyte- and platelet-endothelial cell interactions.
|
| Journal |
Am J Physiol Gastrointest Liver Physiol
|
| Abstract |
Recent findings indicate that dextran sodium sulfate (DSS)-induced colitis is associated with a prothrombogenic phenotype, with P-selectin playing a major role in platelet recruitment. It has been suggested that probiotics may ameliorate colonic inflammation. We therefore investigated how treatment with Lactobacillus reuteri influenced P-selectin expression, leukocyte and platelet endothelial cell interactions, and colitis severity in DSS-treated rats. Rats were divided into the following four groups: nontreated, DSS treated (5% in drinking water for 9 days), L. reuteri, and L. reuteri and DSS treated. The rats were anesthetized with Inactin (120 mg/kg ip), and the dual radiolabeled monoclonal antibody technique was used to quantify P-selectin expression. Leukocyte-endothelial and platelet-endothelial cell interactions were studied in colonic venules with intravital microscopy. Colitis severity was assessed using a disease activity index. Disease activity index increased, as did the expression of P-selectin in the entire colon after DSS treatment, but both were reduced to control levels with L. reuteri pretreatment. The increased platelet- and leukocyte-endothelial cell interactions after DSS treatment were abolished by pretreatment with L. reuteri. L. reuteri protects against DSS-induced colitis in rats. The protection is associated with reduced P-selectin expression and a decrease in leukocyte- and platelet-endothelial cell interactions.
|
| Volume |
296(3)
|
| Pages |
G534-42
|
| Published |
2009-3-1
|
| DOI |
10.1152/ajpgi.90470.2008
|
| PII |
90470.2008
|
| PMID |
19147805
|
| MeSH |
Animals
Blood Platelets / cytology
Blood Platelets / metabolism
Cell Communication / physiology*
Colitis / pathology
Colitis / prevention & control*
Endothelial Cells / cytology
Endothelial Cells / metabolism
Intestinal Mucosa / metabolism
Intestinal Mucosa / pathology
Leukocytes / cytology
Leukocytes / metabolism
Limosilactobacillus reuteri*
Male
Microscopy, Video
P-Selectin / metabolism*
Probiotics / pharmacology*
Rats
Rats, Sprague-Dawley
|
| IF |
3.725
|
| Times Cited |
36
|
|
WOS Category
|
PHYSIOLOGY
GASTROENTEROLOGY & HEPATOLOGY
|
| Resource |
| General Microbes |
JCM 5869 |
