RRC ID |
11199
|
著者 |
Schreiber O, Petersson J, Phillipson M, Perry M, Roos S, Holm L.
|
タイトル |
Lactobacillus reuteri prevents colitis by reducing P-selectin-associated leukocyte- and platelet-endothelial cell interactions.
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ジャーナル |
Am J Physiol Gastrointest Liver Physiol
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Abstract |
Recent findings indicate that dextran sodium sulfate (DSS)-induced colitis is associated with a prothrombogenic phenotype, with P-selectin playing a major role in platelet recruitment. It has been suggested that probiotics may ameliorate colonic inflammation. We therefore investigated how treatment with Lactobacillus reuteri influenced P-selectin expression, leukocyte and platelet endothelial cell interactions, and colitis severity in DSS-treated rats. Rats were divided into the following four groups: nontreated, DSS treated (5% in drinking water for 9 days), L. reuteri, and L. reuteri and DSS treated. The rats were anesthetized with Inactin (120 mg/kg ip), and the dual radiolabeled monoclonal antibody technique was used to quantify P-selectin expression. Leukocyte-endothelial and platelet-endothelial cell interactions were studied in colonic venules with intravital microscopy. Colitis severity was assessed using a disease activity index. Disease activity index increased, as did the expression of P-selectin in the entire colon after DSS treatment, but both were reduced to control levels with L. reuteri pretreatment. The increased platelet- and leukocyte-endothelial cell interactions after DSS treatment were abolished by pretreatment with L. reuteri. L. reuteri protects against DSS-induced colitis in rats. The protection is associated with reduced P-selectin expression and a decrease in leukocyte- and platelet-endothelial cell interactions.
|
巻・号 |
296(3)
|
ページ |
G534-42
|
公開日 |
2009-3-1
|
DOI |
10.1152/ajpgi.90470.2008
|
PII |
90470.2008
|
PMID |
19147805
|
MeSH |
Animals
Blood Platelets / cytology
Blood Platelets / metabolism
Cell Communication / physiology*
Colitis / pathology
Colitis / prevention & control*
Endothelial Cells / cytology
Endothelial Cells / metabolism
Intestinal Mucosa / metabolism
Intestinal Mucosa / pathology
Lactobacillus reuteri*
Leukocytes / cytology
Leukocytes / metabolism
Male
Microscopy, Video
P-Selectin / metabolism*
Probiotics / pharmacology*
Rats
Rats, Sprague-Dawley
|
IF |
3.725
|
引用数 |
36
|
WOS 分野
|
PHYSIOLOGY
GASTROENTEROLOGY & HEPATOLOGY
|
リソース情報 |
一般微生物 |
JCM 5869 |