RRC ID 11205
Author Iwabuchi N, Takahashi N, Xiao JZ, Yonezawa S, Yaeshima T, Iwatsuki K, Hachimura S.
Title Suppressive effects of Bifidobacterium longum on the production of Th2-attracting chemokines induced with T cell-antigen-presenting cell interactions.
Journal FEMS Immunol. Med. Microbiol.
Abstract In human trials, Bifidobacterium longum BB536 alleviates subjective symptoms of Japanese cedar pollinosis, an IgE-mediated type I allergy caused by exposure to Japanese cedar, and significantly suppresses the increase of plasma thymus- and activation-regulated chemokine (TARC) associated with pollen dispersion. In the present study, we investigated the suppressive effects of BB536 on the production of T helper type 2 (Th2)-attracting chemokines, such as TARC and macrophage-derived chemokine (MDC), together with the mechanisms of their production. Murine splenocytes were cultured with heat-killed BB536, and the levels of Th2-attracting chemokines in the supernatants were measured. TARC and MDC were produced in cultures without stimulation, and the production was significantly suppressed by BB536. These chemokines were produced by antigen-presenting cells (APCs) of splenocytes stimulated with an anti-CD40 antibody. Furthermore, TARC production was induced with granulocyte macrophage colony-stimulating factor that was produced by T cells and dendritic cells. BB536 suppressed MDC production induced with the anti-CD40 antibody by APCs from the spleen, mesenteric lymph nodes (MLNs) and Peyer's patches, and it suppressed TARC production by APCs from the spleen and MLNs. These results indicate that BB536 suppresses the production of Th2-attracting chemokines induced by the T cell-APC interaction, suggesting a novel mechanism for alleviating symptoms of allergic disorders by probiotics.
Volume 55(3)
Pages 324-34
Published 2009-4
DOI 10.1111/j.1574-695X.2008.00510.x
PII FIM510
PMID 19291170
MeSH Animals Antigen-Presenting Cells / immunology* Antigen-Presenting Cells / microbiology* Bifidobacterium / immunology* Cells, Cultured Chemokine CCL17 / antagonists & inhibitors* Chemokine CCL17 / biosynthesis* Chemokine CCL22 / antagonists & inhibitors* Chemokine CCL22 / biosynthesis* Lymph Nodes / immunology Mice Mice, Inbred BALB C Peyer's Patches / immunology Probiotics / pharmacology Spleen / immunology
Times Cited 11
WOS Category INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY
Resource
General Microbes JCM 5803 JCM 1649 JCM 11019