| RRC ID |
11225
|
| 著者 |
Tsuruoka C, Furusawa Y, Anzai K, Okayasu R, Suzuki M.
|
| タイトル |
Rejoining kinetics of G1-PCC breaks induced by different heavy-ion beams with a similar LET value.
|
| ジャーナル |
Mutat Res
|
| Abstract |
In previous studies we have shown that the linear energy transfer (LET)-relative biological effectiveness (RBE) curves were affected by LET and ion species [1,2]. In this paper we have examined the difference in the repair kinetics of G1-prematurely condensed chromosome breaks in normal human fibroblasts following irradiation with different heavy-ion beams of similar LET values. Normal human fibroblasts were irradiated with about 110 keV/microm of carbon (135 MeV/n), neon (400 MeV/n) and silicon ions (490 MeV/n), and the doses of carbon (3.25 Gy), neon (2.94+/-0.01 Gy) and silicon (2.31 Gy) were chosen to produce approximately the same number of initially measured G1-premature chromosome condensation (PCC) breaks (about 37 excess fragments per cell). The number of G1-PCC breaks was counted as excess fragments of prematurely condensed chromosomes using the PCC technique in the G1/G0 phase. The fractions of residual G1-PCC breaks after 24 h post-irradiation and half time, which is the time point where 50% of initially measured G1-PCC breaks are rejoined (t1/2), of the slow components of rejoining in carbon- and neon-ion irradiated cells were different from those of silicon-ion irradiated cells. However, no difference was observed in the half time of the fast components of rejoining in each ion beam. The results suggest that the difference in the fractions of residual G1-PCC breaks after 24 h post-irradiation reflect the result of the slow repair process for induced G1-PCC breaks, and that the repair process is dependent on the ion species, not the LET values.
|
| 巻・号 |
701(1)
|
| ページ |
47-51
|
| 公開日 |
2010-8-14
|
| DOI |
10.1016/j.mrgentox.2010.04.020
|
| PII |
S1383-5718(10)00161-0
|
| PMID |
20420934
|
| MeSH |
Chromosome Aberrations*
DNA Repair
Fibroblasts / radiation effects
G1 Phase
Heavy Ions / adverse effects*
Humans
Linear Energy Transfer*
|
| IF |
2.463
|
| 引用数 |
5
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
TOXICOLOGY
GENETICS & HEREDITY
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
