RRC ID 11241
Author Tateno M, Toyooka M, Shikano Y, Takeda S, Kuwabara N, Sezutsu H, Tamura T.
Title Production and characterization of the recombinant human mu-opioid receptor from transgenic silkworms.
Journal J. Biochem.
Abstract The production of useful quantities of G protein-coupled receptors is a major problem not only for screening of various drug compounds but also in performing structural biology studies. To solve this problem, we investigated the possibility of using transgenic silkworms for the production of these receptors. Using the human mu-opioid receptor gene, we constructed three transgenic silkworm strains that produced mu-opioid receptors. The silkworms expressed significant amounts of the receptor in the fat body and silk gland. The product was evaluated using a saturation ligand-binding assay. The expressed receptor exhibited ligand affinity similar to that of an authentic sample, and the yield from the transgenic silkworm was comparable to that obtained using an Sf9-baculovirus expression system. As the mass rearing of transgenic silkworms has already been established, the silkworms can be adapted for production of large quantities of receptors.
Volume 145(1)
Pages 37-42
Published 2009-1
DOI 10.1093/jb/mvn147
PII mvn147
PMID 18984628
MeSH Animals Animals, Genetically Modified / metabolism Baculoviridae / metabolism Bombyx / genetics* Humans Models, Genetic Receptors, Opioid, mu / biosynthesis* Receptors, Opioid, mu / genetics* Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics
IF 2.35
Times Cited 18
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms NA