| RRC ID |
11247
|
| Author |
Katsuma S, Kang W, Shin-i T, Ohishi K, Kadota K, Kohara Y, Shimada T.
|
| Title |
Mass identification of transcriptional units expressed from the Bombyx mori nucleopolyhedrovirus genome.
|
| Journal |
J Gen Virol
|
| Abstract |
In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from Bombyx mori NPV (BmNPV)-infected BmN cells. We randomly sequenced 11,520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.
|
| Volume |
92(Pt 1)
|
| Pages |
200-3
|
| Published |
2011-1-1
|
| DOI |
10.1099/vir.0.025908-0
|
| PII |
vir.0.025908-0
|
| PMID |
20881086
|
| MeSH |
Animals
Bombyx / virology
Cell Line
DNA, Viral / chemistry
DNA, Viral / genetics
Gene Expression Profiling / methods*
Gene Expression Regulation, Viral
Gene Library
Genome, Viral*
Molecular Sequence Data
Nucleopolyhedroviruses / genetics*
Nucleopolyhedroviruses / growth & development*
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
RNA, Untranslated / biosynthesis
RNA, Untranslated / genetics
RNA, Viral / biosynthesis
RNA, Viral / genetics
Sequence Analysis, DNA
|
| IF |
3.376
|
| Times Cited |
17
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
|
| Resource |
| Silkworms |
cDNA clones |
