論文 - 詳細
| RRC ID | 11249 |
|---|---|
| 著者 | Nakanishi T, Shimada T, Katsuma S. |
| タイトル | Characterization of a Bombyx mori nucleopolyhedrovirus mutant lacking both fp25K and p35. |
| ジャーナル | Virus Genes |
| Abstract |
Recent studies have shown that dual mutations in fp25K and p35 of Autographa californica nucleopolyhedrovirus (AcMNPV) result in a typical apoptotic infection on Trichoplusia ni cells, suggesting the involvement of FP25K on NPV-induced apoptosis. To examine the effect of fp25K deletion on Bombyx mori NPV (BmNPV)-induced apoptosis, we generated a BmNPV mutant, fp-p35D, in which both fp25K and p35 genes are deleted from the genome, and compared its phenotype with wild-type (T3), fp25K-deleted (fp-null), and p35-deleted (p35D) BmNPVs. In BmN cells, p35D, but not T3 or fp-null, caused apoptosis with caspase-3 activation. Infection with fp-p35D also resulted in caspase-3 activation, but the level was comparable to that of p35D. Also, we did not observe any apoptotic responses in hemocytes from larvae infected with p35D or fp-p35D. These results indicate that unlike AcMNPV, deletion of fp25K does not affect the pathway of p35D-induced apoptosis of BmN cells and B. mori larvae. |
| 巻・号 | 41(1) |
| ページ | 144-8 |
| 公開日 | 2010-8-1 |
| DOI | 10.1007/s11262-010-0492-5 |
| PMID | 20508980 |
| MeSH | Animals Bombyx / virology Gene Deletion* Genome, Viral Larva / virology Mutation* Nucleopolyhedroviruses / genetics Nucleopolyhedroviruses / metabolism* Viral Proteins / genetics Viral Proteins / metabolism* |
| IF | 1.991 |
| 引用数 | 2 |
| WOS 分野 | GENETICS & HEREDITY VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
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