論文 - 詳細
| RRC ID | 11256 |
|---|---|
| 著者 | Kawaoka S, Hayashi N, Katsuma S, Kishino H, Kohara Y, Mita K, Shimada T. |
| タイトル | Bombyx small RNAs: genomic defense system against transposons in the silkworm, Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
Selfish genetic elements called transposons can insert themselves at new locations in host genomes to modify gene structure and alter gene expression. Expansion of transposons can occur when novel transposition events are transmitted to subsequent generations after germline hopping. Therefore, organisms seem likely to have evolved defense mechanisms to silence transposons in the germline. Recently, small RNAs interacting with Piwi proteins (piwi-interacting RNAs: piRNAs) have been demonstrated to be involved in genomic defense mechanism against transposons. Here, we show that piRNA-like small RNAs are present abundantly in the Bombyx ovary. We cloned 38,493 kinds of Bombyx small RNA from the ovary and performed functional characterization. Bombyx small RNAs showed a unimodal length distribution with a peak at 28nt and a strong bias for U at the 5' end. We found that 12,869 kinds of Bombyx small RNAs were associated with transposons or repetitive sequences. We classified them as repeat-associated small interfering RNAs (rasiRNAs), a subclass of piRNAs. Notably, antisense rasiRNAs have a strong bias toward U at 5' ends; in contrast, sense rasiRNAs have a strong bias toward A at nucleotide position 10, indicating that the piRNA amplification loop proposed in Drosophila is evolutionarily conserved in Bombyx. These results suggest that Bombyx small RNAs regulate transposon activity. |
| 巻・号 | 38(12) |
| ページ | 1058-65 |
| 公開日 | 2008-12-1 |
| DOI | 10.1016/j.ibmb.2008.03.007 |
| PII | S0965-1748(08)00045-3 |
| PMID | 18801438 |
| MeSH | Animals Bombyx / genetics* Cloning, Molecular DNA Transposable Elements / genetics* Gene Silencing RNA, Small Interfering / classification RNA, Small Interfering / genetics* |
| IF | 3.827 |
| 引用数 | 33 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | NA |