論文 - 詳細
RRC ID | 11260 |
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著者 | Yamamoto K, Aso Y, Fujii H. |
タイトル | Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori. |
ジャーナル | J Insect Sci |
Abstract |
The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and specifically to MCM7, of vertebrates and invertebrates. An RT-PCR showed that the bmMCM7 transcript was present in the ovaries, testes, silk glands, and fat bodies of larval silkworms. Expression plasmids were transformed into competent Escherichia coli and overexpressed. This is the first report on the identification of MCM helicase of the silkworm, B. mori. |
巻・号 | 10 |
ページ | 148 |
公開日 | 2010-1-1 |
DOI | 10.1673/031.010.14108 |
PMID | 21070176 |
PMC | PMC3016899 |
MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Cloning, Molecular Cluster Analysis DNA Helicases / genetics* DNA Primers / genetics DNA, Complementary / genetics Escherichia coli Genetic Vectors / genetics Molecular Sequence Data Nuclear Proteins / genetics* Phylogeny* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity |
IF | 1.325 |
引用数 | 0 |
WOS 分野 | ENTOMOLOGY |
リソース情報 | |
カイコ | p50 |