RRC ID 11262
著者 Royer C, Briolay J, Garel A, Brouilly P, Sasanuma S, Sasanuma M, Shimomura M, Keime C, Gandrillon O, Huang Y, Chavancy G, Mita K, Couble P.
タイトル Novel genes differentially expressed between posterior and median silk gland identified by SAGE-aided transcriptome analysis.
ジャーナル Insect Biochem Mol Biol
Abstract Serial analysis of gene expression (SAGE) profiles, from posterior and median cells of the silk gland of Bombyx mori, were analyzed and compared, so as to identify their respective distinguishing functions. The annotation of the SAGE libraries was performed with a B. mori reference tag collection, which was extracted from a novel set of Bombyx ESTs, sequenced from the 3' side. Most of the tags appeared at similar relative concentration within the two libraries, and corresponded with region-specific and highly abundant silk proteins. Strikingly, in addition to tags from silk protein mRNAs, 19 abundant tags were found (≥ 0.1%), in the median cell library, which were absent in the posterior cell tag collection. With the exception of tags from SP1 mRNA, no PSG specific tags were found in this subset class. The analysis of some of the MSG-specific transcripts, suggested that middle silk gland cells have diversified functions, in addition to their well characterized role in silk sericins synthesis and secretion.
巻・号 41(2)
ページ 118-24
公開日 2011-2-1
DOI 10.1016/j.ibmb.2010.11.003
PII S0965-1748(10)00239-0
PMID 21078388
MeSH Animals Base Sequence Bombyx / genetics* Expressed Sequence Tags Female Fibroins / genetics* Gene Expression Gene Expression Profiling* Gene Library Genes, Insect Molecular Sequence Data Multigene Family RNA, Messenger Sequence Analysis Sericins / genetics*
IF 3.827
引用数 11
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ NA