| RRC ID |
11265
|
| 著者 |
Nomura T, Ikeda M, Ishiyama S, Mita K, Tamura T, Okada T, Fujiyama K, Usami A.
|
| タイトル |
Cloning and characterization of a β-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
In insects, β-N-acetylglucosaminidase (GlcNAcase) participates in critical physiological processes such as fertilization, metamorphosis, and glycoconjugate degradation. Insects produce glycoproteins carrying paucimannosidic-type N-glycans, the terminal GlcNAc residue of which is cleaved by a GlcNAc-linkage specific GlcNAcase, also known as the fused lobes (FDL) protein. To obtain information on the structure of GlcNAcases and insight into their contribution to physiological processes, we cloned Bombyx mori FDL (BmFDL) from silkworm larvae. The full-length cDNA (1.9 kb) encoded a protein of 633 amino acids with 42% amino acid sequence identity to Drosophila melanogaster FDL (DmFDL). Recombinant BmFDL cleaved only β-1,2-linked GlcNAc residues from the α-1,3 branch of biantennary N-glycan. This substrate specificity was similar to that of DmFDL. Microsomal FDL activity was inhibited by anti-BmFDL antibodies. Taken together, our results suggest that BmFDL is a N-glycan-processing GlcNAcase in B. mori.
|
| 巻・号 |
110(4)
|
| ページ |
386-91
|
| 公開日 |
2010-10-1
|
| DOI |
10.1016/j.jbiosc.2010.04.008
|
| PII |
S1389-1723(10)00143-X
|
| PMID |
20547376
|
| MeSH |
Acetylglucosaminidase / chemistry
Acetylglucosaminidase / genetics
Acetylglucosaminidase / metabolism*
Animals
Base Sequence
Bombyx / enzymology*
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Cloning, Molecular
DNA Primers
DNA, Complementary
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
|
| IF |
2.366
|
| 引用数 |
15
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| カイコ |
NA |
