RRC ID |
11266
|
著者 |
Tsubota T, Shimomura M, Ogura T, Seino A, Nakakura T, Mita K, Shinoda T, Shiotsuki T.
|
タイトル |
Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori.
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ジャーナル |
Insect Biochem Mol Biol
|
Abstract |
We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholinesterase (CCE) genes having GQSAG, a highly conserved motif in JHE, by the use of silkworm genomic database. Five novel CCE genes (Bmcce-1-5) were identified and their cDNA sequences and intron-exon structures were determined. We investigated the developmental expression patterns of these CCE genes by real-time quantitative PCR analysis and found that their expression patterns varied among developmental stages and organs. Of the proteins produced by the five genes, only BmCCE-5 had the ability to degrade JH; however, this protein might not function as a JH-specific esterase in vivo as it had a high K(m) value for JH. On the other hand, BmCCE-5 degraded general esterase substrates efficiently. Since Bmcce-5 was strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism. Our results suggest that of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm.
|
巻・号 |
40(2)
|
ページ |
100-12
|
公開日 |
2010-2-1
|
DOI |
10.1016/j.ibmb.2009.12.015
|
PII |
S0965-1748(09)00195-7
|
PMID |
20060470
|
MeSH |
Amino Acid Motifs*
Amino Acid Sequence
Animals
Base Sequence
Bombyx / enzymology*
Carboxylic Ester Hydrolases / chemistry
Carboxylic Ester Hydrolases / genetics*
Carboxylic Ester Hydrolases / metabolism
Cholinesterases / chemistry
Cholinesterases / genetics*
Cholinesterases / metabolism
Cloning, Molecular
DNA Primers
Exons
Introns
Kinetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
IF |
3.827
|
引用数 |
28
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
カイコ |
NA |