論文 - 詳細
| RRC ID | 11266 |
|---|---|
| 著者 | Tsubota T, Shimomura M, Ogura T, Seino A, Nakakura T, Mita K, Shinoda T, Shiotsuki T. |
| タイトル | Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholinesterase (CCE) genes having GQSAG, a highly conserved motif in JHE, by the use of silkworm genomic database. Five novel CCE genes (Bmcce-1-5) were identified and their cDNA sequences and intron-exon structures were determined. We investigated the developmental expression patterns of these CCE genes by real-time quantitative PCR analysis and found that their expression patterns varied among developmental stages and organs. Of the proteins produced by the five genes, only BmCCE-5 had the ability to degrade JH; however, this protein might not function as a JH-specific esterase in vivo as it had a high K(m) value for JH. On the other hand, BmCCE-5 degraded general esterase substrates efficiently. Since Bmcce-5 was strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism. Our results suggest that of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm. |
| 巻・号 | 40(2) |
| ページ | 100-12 |
| 公開日 | 2010-2-1 |
| DOI | 10.1016/j.ibmb.2009.12.015 |
| PII | S0965-1748(09)00195-7 |
| PMID | 20060470 |
| MeSH | Amino Acid Motifs* Amino Acid Sequence Animals Base Sequence Bombyx / enzymology* Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / genetics* Carboxylic Ester Hydrolases / metabolism Cholinesterases / chemistry Cholinesterases / genetics* Cholinesterases / metabolism Cloning, Molecular DNA Primers Exons Introns Kinetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid |
| IF | 3.827 |
| 引用数 | 28 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カイコ | NA |